Castillo M C, Tapia F J, Arciniegas E
Laboratorio de Biologia Molecular, Universidad Central de Venezuela.
J Med Vet Mycol. 1990;28(1):91-4. doi: 10.1080/02681219080000111.
The ultrastructural localization of antigens recognized by an antiserum raised against formaline-killed Sporothrix schenckii yeast cells was investigated on yeast and mycelial phases of the fungus. Immunogold procedures revealed that these antigens were located on the cell surface of both growth phases. Labelling was heterogeneous and involved areas of Concanavalin A-binding sugar residues.
利用抗福尔马林灭活申克孢子丝菌酵母细胞制备的抗血清,对该真菌的酵母相和菌丝相进行了抗原的超微结构定位研究。免疫金法显示,这些抗原位于两个生长阶段的细胞表面。标记是不均匀的,且涉及伴刀豆球蛋白A结合糖残基的区域。