申克孢子丝菌细胞表面伴刀豆球蛋白A结合抗原的鉴定

Identification of a concanavalin A-binding antigen of the cell surface of Sporothrix schenckii.

作者信息

Lima O C, Bezerra L M

机构信息

Departamento de Biologia Celular e Genética, Universidade do Estado do Rio de Janeiro, Brasil.

出版信息

J Med Vet Mycol. 1997 May-Jun;35(3):167-72. doi: 10.1080/02681219780001101.

DOI:10.1080/02681219780001101

PMID:9229332

Abstract

Sporothrix schenckii (1099-18) cell wall peptido-rhamnomannan (CWPR) was fractionated by affinity chromatography with Concanavalin A. The Con A-bound and Con A-unbound fractions were probed with an anti-S. schenckii rabbit serum. We identified within the Con A-bound fraction three main antigens with approximate molecular weights of 84, 70 and 58 kDa. Glycopeptide beta-elimination reduced rabbit antiserum reactivity for the 84 kDa antigen (gp84) with concomittant enhanced reactivity for the 70 kDa antigen (gp70). By Western blot with Con A-HRP conjugate we demonstrated that gp84 strongly reacted with this lectin and this was the predominant antigen identified. The gp84 antigen was also demonstrated to be present on other S. schenckii strains.

摘要

申克孢子丝菌（1099 - 18）的细胞壁肽甘露聚糖（CWPR）通过伴刀豆球蛋白A亲和层析进行分级分离。用抗申克孢子丝菌兔血清检测伴刀豆球蛋白A结合和未结合的部分。我们在伴刀豆球蛋白A结合部分中鉴定出三种主要抗原，其近似分子量分别为84、70和58 kDa。糖肽β-消除降低了兔抗血清对84 kDa抗原（gp84）的反应性，同时增强了对70 kDa抗原（gp70）的反应性。通过用伴刀豆球蛋白A - 辣根过氧化物酶缀合物进行蛋白质印迹分析，我们证明gp84与该凝集素强烈反应，并且这是鉴定出的主要抗原。还证明gp84抗原存在于其他申克孢子丝菌菌株上。