Nofer Institute of Occupational Medicine, Department of Toxicology and Carcinogenesis, Poland.
Int J Hyg Environ Health. 2014 Jan;217(1):11-6. doi: 10.1016/j.ijheh.2013.02.009. Epub 2013 Apr 3.
The aim of this study was to design the optimized laboratory protocol as a tool for human biomonitoring of selected Persistent Organic Pollutants (POPs) in Poland. In this study, we present the method developed for the determination of 29 congeners of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and dioxin-like polychlorinated biphenyls (dl-PCBs), as well as individual results of the measurements in 40 human breast milk samples collected in central Poland in 2008-2010.
The protocol of sample preparation and quantitative analysis of PCDD/Fs and dl-PCBs was optimized for the isotopic dilution method with high resolution gas chromatography/high resolution mass spectrometry HRGC/HRMS. Fat content in the extracts was determined gravimetrically. The results were corrected by fat content in the samples.
The average sum of PCDD/F and dl-PCBs in the human milk samples from the urban area was 7.429 WHO-TEQpg/g fat (with the range 0.431-14.27), and in the rural area it was 6.448pg WHO-TEQ/g fat (0.539-12.61).
The results obtained in this study indicate that the mothers were exposed uniformly to PCDD/Fs and PCBs regardless of location. The significant difference of p<0.1 between the milk samples from the urban and rural mothers in 2,3,7,8-TCDD; 1,2,37,8,9-HxCDD and 2,3,4,6,7-HxCDF were observed. For the other 14 PCDD/F and 12 dl-PCB congeners, the observed differences were not significant. The total WHO-TEQ values are lower in comparison with the average results in Europe from the fourth round of a WHO-coordinated study.
本研究旨在设计优化的实验室方案，作为波兰特定持久性有机污染物（POPs）人体生物监测的工具。在本研究中，我们提出了一种用于测定 29 种多氯二苯并对二恶英（PCDDs）、多氯二苯并呋喃（PCDFs）和类二恶英多氯联苯（dl-PCBs）同系物的方法，以及 2008 年至 2010 年在波兰中部采集的 40 个人乳样本中测量的个别结果。
使用高分辨率气相色谱/高分辨率质谱（HRGC/HRMS）同位素稀释法优化了 PCDD/Fs 和 dl-PCBs 的样品制备和定量分析方案。提取物中的脂肪含量通过重量法测定。结果通过样品中的脂肪含量进行校正。
城市地区人乳样本中 PCDD/F 和 dl-PCBs 的平均总和为 7.429 世卫组织-TEQpg/g 脂肪（范围为 0.431-14.27），农村地区为 6.448pg 世卫组织-TEQ/g 脂肪（0.539-12.61）。
本研究结果表明，无论地点如何，母亲都均匀暴露于 PCDD/Fs 和 PCBs 中。在城市和农村母亲的奶样中，2,3,7,8-TCDD、1,2,37,8,9-HxCDD 和 2,3,4,6,7-HxCDF 之间观察到 p<0.1 的显著差异。对于其他 14 种 PCDD/F 和 12 种 dl-PCB 同系物，观察到的差异不显著。与世界卫生组织协调的第四轮研究中欧洲的平均结果相比，总世卫组织-TEQ 值较低。
