School of Life Science, Liaoning Normal University, Dalian 116081, China.
Bioresour Technol. 2013 Jun;138:307-13. doi: 10.1016/j.biortech.2013.03.183. Epub 2013 Apr 3.
The aim of this work was to investigate the decolorization and degradation of azo dyes by growing cells of a new yeast strain TL-F1 which was isolated from the sea mud. Strain TL-F1 was identified as Candida tropicalis on the basis of 28S rDNA analysis. Various azo dyes (20mg/L) were efficiently decolorized through aerobic degradation. Meantime, the effects of different parameters on both decolorization of Acid Brilliant Scarlet GR and growth of strain TL-F1 were investigated. Furthermore, possible degradation pathway of the dye GR was proposed through analysis of metabolic products using UV-Vis spectroscopy and HPLC-MS methods. As far as it is known, it is the first systematic research on a C. tropicalis strain which is capable of efficiently decolorizing various azo dyes under aerobic condition. This work provides a potentially useful microbial strain TL-F1 for treatment of azo dye contaminated wastewater.
本工作旨在研究从海泥中分离得到的一株新型酵母 TL-F1 细胞对偶氮染料的脱色和降解作用。根据 28S rDNA 分析，菌株 TL-F1 被鉴定为热带假丝酵母。该菌株在好氧条件下通过有氧降解能有效地对各种偶氮染料（20mg/L）进行脱色。同时，考察了不同参数对偶氮染料酸性艳红 GR 的脱色和菌株 TL-F1 生长的影响。此外，通过利用紫外-可见光谱和高效液相色谱-质谱联用（HPLC-MS）方法分析代谢产物，提出了染料 GR 的可能降解途径。据所知，这是首次对在好氧条件下能有效降解各种偶氮染料的热带假丝酵母菌株进行系统研究。这项工作为处理偶氮染料污染废水提供了一种潜在有用的微生物菌株 TL-F1。
