Franco's Jack-in-the-pulpit and Waxy Jack-in-the-pulpit essential oils are active against Haemonchus contortus.
Anthelmintic activity of Arisaema franchetianum and Arisaema lobatum essential oils against Haemonchus contortus.
机构信息
College of Food and Bioengineering, South China University of Technology, Guangzhou, Guangdong Province, China.
出版信息
J Ethnopharmacol. 2013 Jun 21;148(1):311-6. doi: 10.1016/j.jep.2013.04.034. Epub 2013 Apr 25.
ETHNOPHARMACOLOGICAL RELEVANCE
Arisaema franchetianum and Arisaema lobatum are two perennial plants native to China. Arisaema franchetianum is universally used to promote the subsidence of induration and swelling, quicken blood and relieve pains, and kill intestinal parasites in humans and animals. Arisaema lobatum is used to treat malaria, intestinal parasites, and snake and insect bites in humans and animals. The aim of this study was to determine the composition of the essential oils from Arisaema franchetianum and Arisaema lobatum and evaluate the anthelmintic effect against Haemonchus contortus.
MATERIALS AND METHODS
Two oils were investigated by GC and GC-MS. The anthelmintic bioassay tests of Arisaema franchetianum and Arisaema lobatum essential oil, linalool and carvacrol were performed using egg hatch assay (EHA), larval development assay (LDA) and larval migration inhibition assay (LMIA).
RESULTS
Fifty six components representing 96.88% of the Arisaema franchetianum oil and 64 components representing 96.88% of the Arisaema lobatum oil were identified. Carvacrol and linalool were found to be the major constituents of two oils. In the EHA, greater than 99% inhibition were observed with Arisaema franchetianum oil at 10mg/mL (CE50 1.63mg/mL) and Arisaema lobatum oil at 5 and 10mg/mL (CE50 0.48mg/mL). In the LDA, both oils induced complete inhibition at 10mg/mL, with the CE50 being 1.10mg/mL for Arisaema franchetianum oil and 0.73mg/mL for Arisaema lobatum oil. In the LMIA, the Arisaema franchetianum oil and Arisaema lobatum oil at best inhibited 74.1% and 95.6% of larval migration at 10mg/mL, respectively. Carvacrol exhibited similar activity to Arisaema lobatum essential oil and linalool did not show high activity in every assay.
CONCLUSIONS
These data show for the first time that the essential oils obtained from Arisaema franchetianum or Arisaema lobatum had promising anthelmintic activity against Haemonchus contortus. Arisaema plant may offer an alternative source for the control of gastrointestinal nematodes of sheep and goats.
民族药理学相关性
佛焰苞芋和芋艿是两种原产于中国的多年生植物。佛焰苞芋在临床上被广泛用于治疗人类和动物的硬结和肿胀、促进血液流动和缓解疼痛、以及杀灭肠道寄生虫；芋艿被用于治疗人类和动物的疟疾、肠道寄生虫以及蛇虫咬伤。本研究旨在确定佛焰苞芋和芋艿精油的组成，并评估其对捻转血矛线虫的驱虫效果。
材料与方法
采用 GC 和 GC-MS 对两种精油进行了研究。采用卵孵化试验（EHA）、幼虫发育试验（LDA）和幼虫迁移抑制试验（LMIA）对佛焰苞芋和芋艿精油、芳樟醇和香芹酚进行了驱虫生物测定试验。
结果
鉴定出佛焰苞芋油中 56 种成分，占 96.88%，芋艿油中 64 种成分，占 96.88%。发现香芹酚和芳樟醇是两种油的主要成分。在 EHA 中，佛焰苞芋油在 10mg/mL 时的抑制率大于 99%（CE50 为 1.63mg/mL），芋艿油在 5 和 10mg/mL 时的抑制率大于 99%（CE50 分别为 0.48mg/mL 和 0.73mg/mL）。在 LDA 中，两种油在 10mg/mL 时均能完全抑制幼虫发育，CE50 分别为 1.10mg/mL 和 0.73mg/mL。在 LMIA 中，佛焰苞芋油和芋艿油在 10mg/mL 时对幼虫迁移的最大抑制率分别为 74.1%和 95.6%。香芹酚表现出与芋艿油相似的活性，而芳樟醇在每种试验中均未表现出高活性。
结论
这些数据首次表明，佛焰苞芋或芋艿精油具有良好的抗捻转血矛线虫活性。阿萨伊属植物可能为控制绵羊和山羊的胃肠道线虫提供了一种替代来源。
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