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剑麻渣中一种新多糖(SP1)的结构特征、抗氧化和免疫活性。

Structural features, antioxidant and immunological activity of a new polysaccharide (SP1) from sisal residue.

机构信息

College of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, PR China.

出版信息

Int J Biol Macromol. 2013 Aug;59:184-91. doi: 10.1016/j.ijbiomac.2013.04.052. Epub 2013 Apr 24.

DOI:10.1016/j.ijbiomac.2013.04.052
PMID:23624283
Abstract

A new water-soluble polysaccharide (SP1) with molecular weight of 9192Da was isolated from the residue after extracting fiber from sisal leaf and its structure was investigated. Monosaccharide composition analysis showed that SP1 was composed of Rha, GalA, Gal, Ara and Glc in a molar ratio of 1.82:1.69:1.00:0.23:0.15. The structure analysis indicated that the SP1 was comprised of a backbone of alternating 1,2-linked-α-l-Rhap and 1,4-linked-α-d-GalpA units, and the neutral polysaccharide side chains composed of Galp, Ara and Glcp residues were attached to the O-4 position of 1,2-linked-α-l-Rhap residues. SP1 could stimulate ConA-induced T lymphocyte proliferation and had noticeable DPPH radical-scavenging ability.

摘要

从剑麻叶中提取纤维后的残渣中分离得到一种新的水溶性多糖(SP1),其分子量为 9192Da,并对其结构进行了研究。单糖组成分析表明,SP1 由 Rha、GalA、Gal、Ara 和 Glc 组成,摩尔比为 1.82:1.69:1.00:0.23:0.15。结构分析表明,SP1 由交替的 1,2-连接-α-l-Rhap 和 1,4-连接-α-d-GalpA 单元组成的主链,以及由 Galp、Ara 和 Glcp 残基组成的中性多糖侧链连接在 1,2-连接-α-l-Rhap 残基的 O-4 位置上。SP1 可刺激 ConA 诱导的 T 淋巴细胞增殖,具有显著的 DPPH 自由基清除能力。

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