Shahrood University of Technology, Faculty of Agriculture, School of Agricultural Engineering, Shahrood, Iran.
Int J Biol Macromol. 2011 Nov 1;49(4):567-72. doi: 10.1016/j.ijbiomac.2011.06.012. Epub 2011 Jun 17.
ABPS-1, a new water-soluble polysaccharide with molecular weight of 26 kDa and a specific optical rotation of +170° (c 1.0, H(2)O), was extracted from the roots of Acanthophyllum bracteatum by warm water and further successively purified through DEAE-cellulose A52 and Sephadex G-100 columns. Monosaccharide analysis revealed that the ABPS-1 was composed of Glc, Gal and Ara with a relative molar ratio of 1.4:5.2:1.0. Its structural features were elucidated by a combination of FT-IR, methylation and GC-MS analysis, periodate oxidation and Smith degradation, partial acid hydrolysis and (13)C and (1)H NMR spectroscopy. The data obtained indicate that ABPS-1 possessed a backbone of α-(1→6)-linked Gal with branches attached to O-2 by α-1→linked Glc and at O-3 by α-1→linked Gal and by α-(1→3)-linked Ara. The in vitro antioxidant activity showed that ABPS-1 possesses DPPH radical-scavenging activity in a concentration-dependent manner with an EC(50) value of 2.6 mg/ml.
ABPS-1 是一种新型的水溶性多糖，分子量为 26 kDa，比旋光度为+170°（c 1.0，H(2)O），从黄花草木樨的根部用水提取，然后通过 DEAE-纤维素 A52 和 Sephadex G-100 柱进一步纯化。单糖分析表明，ABPS-1 由 Glc、Gal 和 Ara 组成，相对摩尔比为 1.4:5.2:1.0。通过 FT-IR、甲基化和 GC-MS 分析、过碘酸氧化和 Smith 降解、部分酸水解和 (13)C 和 (1)H NMR 光谱的组合，阐明了其结构特征。所得数据表明，ABPS-1 具有以 α-(1→6)-连接的 Gal 为骨架的结构，分支通过 α-1→连接的 Glc 在 O-2 上连接，通过 α-1→连接的 Gal 在 O-3 上连接，通过 α-(1→3)-连接的 Ara 在 O-4 上连接。体外抗氧化活性表明，ABPS-1 具有 DPPH 自由基清除活性，呈浓度依赖性，EC(50)值为 2.6 mg/ml。
