Cai Jiale, Zhong Xiaoting, Liang Jiayin, Xu Can, Yu Huanzheng, Xian Minghua, Yan Chunyan, Wang Shumei
Guangdong Pharmaceutical University Guangzhou 510006 China
Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006 China.
RSC Adv. 2021 Nov 8;11(57):35994-36006. doi: 10.1039/d1ra07385k. eCollection 2021 Nov 4.
Diabetes seriously endangers public health and brings a heavy economic burden to the country. Inflammation is one of the main inducing factors of type-2 diabetes (T2D) and may cause some complications of diabetes, such as diabetic encephalopathy and peripheral neuropathy. In-depth research and development of drugs to cure diabetes and complications are of great significance. is a medicinal herb used in several countries to treat many diseases. Here, two new polysaccharides (PLB-1-1 and PLB-1-2) were isolated and purified from the root of with molecular weights of 9.1 × 10 Da and 3.8 × 10 Da, respectively. The structure was evaluated by monosaccharide composition, GC-MS and NMR spectroscopy. It was determined that PLB-1-1 comprised →4)-α-d-Glc-(1→, α-d-Glc-(1→, →6)-β-d-Gal-(1→, →3)-α-l-Ara-(1→, →3,6)-β-d-Man-(1→ and →4,6)-β-d-Man-(1→, and PLB-1-2 consisted of →4)-α-d-Glc-(1→, β-d-Glc-(1→, →4,6)-β-d-Glc-(1→, →3,6)-β-d-Man-(1→ and α-l-Fuc-(1→. Furthermore, both PLB-1-1 and PLB-1-2 showed anti-inflammatory and inhibitory activities of α-glucosidase and α-amylase . Therefore, the new polysaccharides, , PLB-1-1 and PLB-1-2, may be considered candidates for the treatment of diabetes and its related complications.
糖尿病严重危害公众健康，给国家带来沉重的经济负担。炎症是2型糖尿病（T2D）的主要诱发因素之一，可能导致糖尿病的一些并发症，如糖尿病性脑病和周围神经病变。深入研发治疗糖尿病及其并发症的药物具有重要意义。 是一种在多个国家用于治疗多种疾病的草药。在此，从 的根部分离并纯化出两种新的多糖（PLB - 1 - 1和PLB - 1 - 2），分子量分别为9.1×10 Da和3.8×10 Da。通过单糖组成、气相色谱 - 质谱联用（GC - MS）和核磁共振光谱（NMR）对其结构进行了评估。确定PLB - 1 - 1由→4)-α - d - Glc-(1→、α - d - Glc-(1→、→6)-β - d - Gal-(1→、→3)-α - l - Ara-(1→、→3,6)-β - d - Man-(1→和→4,6)-β - d - Man-(1→组成，PLB - 1 - 2由→4)-α - d - Glc-(1→、β - d - Glc-(1→、→4,6)-β - d - Glc-(1→、→3,6)-β - d - Man-(1→和α - l - Fuc-(1→组成。此外，PLB - 1 - 1和PLB - 1 - 2均表现出抗炎以及α - 葡萄糖苷酶和α - 淀粉酶抑制活性。因此，新的多糖 、PLB - 1 - 1和PLB - 1 - 2可能被视为治疗糖尿病及其相关并发症的候选药物。
