Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology, University of Granada, Center of Biomedical Research, Laboratory 123, Avenida del Conocimiento s/n, 18016 Armilla, Granada, Spain.
Peptides. 2013 Jul;45:22-7. doi: 10.1016/j.peptides.2013.04.007. Epub 2013 Apr 25.
Variants in the neuropeptide Y (NPY) gene have been associated with obesity and its traits. The objective of the present study was to evaluate the association of single nucleotide polymorphisms (SNPs) in the NPY gene with obesity, metabolic syndrome features, and inflammatory and cardiovascular disease (CVD) risk biomarkers in Spanish children. We recruited 292 obese children and 242 normal-body mass index (BMI) children. Height, weight, BMI, waist circumference, clinical and metabolic markers, adipokines, and inflammatory (PCR, IL-6, IL-8 and TNF-α) and CVD risk biomarkers (MPO, MMP-9, sE-selectin, sVCAM, sICAM, and PAI-1) were analyzed. Seven SNPs in the NPY gene were genotyped. The results of our study indicate that anthropometric measurements, clinical and metabolic markers, adipokines (leptin and resistin), and inflammatory and CVD risk biomarkers were generally elevated in the obese group. The exceptions to this finding included cholesterol, HDL-c, and adiponectin, which were lower in the obese group, and glucose, LDL-c, and MMP-9, which did not differ between the groups. Both rs16147 and rs16131 were associated with the risk of obesity, and the latter was also associated with insulin resistance, triacylglycerols, leptin, and HDL-c. Thus, we confirm the association of rs16147 with obesity, and we demonstrate for the first time the association of rs16131 with obesity and its possible impact on the early onset of metabolic syndrome features, mainly triacylglycerols, in children.
神经肽 Y（NPY）基因中的变异与肥胖及其特征有关。本研究的目的是评估 NPY 基因中单核苷酸多态性（SNPs）与肥胖、代谢综合征特征以及炎症和心血管疾病（CVD）风险生物标志物在西班牙儿童中的相关性。我们招募了 292 名肥胖儿童和 242 名正常体重指数（BMI）儿童。分析了身高、体重、BMI、腰围、临床和代谢标志物、脂肪因子以及炎症（PCR、IL-6、IL-8 和 TNF-α）和 CVD 风险生物标志物（MPO、MMP-9、sE-选择素、sVCAM、sICAM 和 PAI-1）。对 NPY 基因中的 7 个 SNP 进行了基因分型。我们的研究结果表明，肥胖组的人体测量学测量、临床和代谢标志物、脂肪因子（瘦素和抵抗素）以及炎症和 CVD 风险生物标志物通常升高。这种发现的例外包括胆固醇、HDL-c 和脂联素，它们在肥胖组中较低，而葡萄糖、LDL-c 和 MMP-9 在两组之间没有差异。rs16147 和 rs16131 均与肥胖风险相关，后者还与胰岛素抵抗、三酰甘油、瘦素和 HDL-c 相关。因此，我们证实了 rs16147 与肥胖的相关性，并且我们首次证明了 rs16131 与肥胖及其对儿童代谢综合征特征（主要是三酰甘油）早期发病的可能影响之间的相关性。
