Badr Eman A E, El-Aleem Hassan Abd El-Aleem Abd, El-Ghlban Samah, Swelm Asmaa Ah, Emara Mahmoud
Medical Biochemistry and Molecular Biology Department. Faculty of Medicine, Menoufia University, Shebin Elkom City, Menoufia governorate, Egypt.
Department of Organic Chemistry, Faculty of Science, Menoufia University, Egypt.
Biochem Biophys Rep. 2019 Jul 19;19:100666. doi: 10.1016/j.bbrep.2019.100666. eCollection 2019 Sep.
The prognosis of high-risk patients might be greatly ameliorated using genetic predisposition risk factors. Sympathetic activity and innate immunity related to neuropeptide Y function may be related to dyslipidemia and atherosclerosis. The aim of this study is to detect the correlation between Neuropeptide Y (NPY) SNP rs16147 and its gene expression in chronic kidney disease with and without hypertension.
This study carried out on 150 subjects who were divided into 3 main groups group (I) 50 CKD patients with hypertension, group (II) 50 CKD patients without hypertension and group (III) 50 healthy individuals. Carotid intima media thickness (CIMT) was measured by Ultrasound. Kidney function test and lipid profile were performed. Genotyping and gene expression of neuropeptide Y (NPY) were performed using real time PCR.
There was a significant increase in number and percentage of CC genotype and C allele of NPY SNP distribution in CKD patients with and without hypertension when compared to controls. A significant association was found between CC genotype and C allele and the risk of CKD with hypertension with odd ratio 3.26 and 1.77, respectively. There is a significant positive correlation between NPY gene expression level and CIMT among chronic kidney disease patients with highest level of TC, LDLc and CIMT among CC genotype of NPY gene.
A significant association was found between CC genotype and C allele of NPY at rs16147 with increase NPY gene expression and risk of developing hypertension in CKD.
利用遗传易感性风险因素可能会大大改善高危患者的预后。与神经肽Y功能相关的交感神经活动和先天免疫可能与血脂异常和动脉粥样硬化有关。本研究的目的是检测神经肽Y（NPY）单核苷酸多态性rs16147与其基因表达在伴有和不伴有高血压的慢性肾脏病中的相关性。
本研究对150名受试者进行，他们被分为3个主要组：组（I）50名患有高血压的慢性肾脏病患者，组（II）50名不患有高血压的慢性肾脏病患者，组（III）50名健康个体。通过超声测量颈动脉内膜中层厚度（CIMT）。进行肾功能测试和血脂谱分析。使用实时PCR进行神经肽Y（NPY）的基因分型和基因表达分析。
与对照组相比，患有和不患有高血压的慢性肾脏病患者中，NPY单核苷酸多态性分布的CC基因型和C等位基因的数量和百分比显著增加。发现CC基因型和C等位基因与伴有高血压的慢性肾脏病风险之间存在显著关联，比值比分别为3.26和1.77。在NPY基因CC基因型的慢性肾脏病患者中，NPY基因表达水平与CIMT之间存在显著正相关，这些患者的总胆固醇（TC）、低密度脂蛋白胆固醇（LDLc）和CIMT水平最高。
发现rs16147处NPY的CC基因型和C等位基因与慢性肾脏病中NPY基因表达增加及高血压发生风险之间存在显著关联。
