Institut des Biomolécules Max Mousseron (UMR 5247 CNRS-Université de Montpellier 1-Université de Montpellier 2), place Eugène Bataillon CC 1706, 34095 Montpellier Cedex 5, France.
Anal Bioanal Chem. 2013 Jun;405(16):5369-79. doi: 10.1007/s00216-013-6972-4. Epub 2013 Apr 28.
In this work, Taylor dispersion analysis and capillary electrophoresis were used to characterize the size and charge of polymeric drug delivery nanogels based on polyglutamate chains grafted with hydrophobic groups of vitamin E. The hydrophobic vitamin E groups self-associate in water to form small hydrophobic nanodomains that can incorporate small drugs or therapeutic proteins. Taylor dispersion analysis is well suited to determine the weight average hydrodynamic radius of nanomaterials and to get information on the size polydispersity of polymeric samples. The effective charge was determined either from electrophoretic mobility and hydrodynamic radius using electrophoretic modeling (three different approaches were compared), or by indirect UV detection in capillary electrophoresis. The influence of vitamin E hydrophobicity on the polymer effective charge has been studied. The presence of vitamin E leads to a drastic decrease in polymer effective charge in comparison to non-modified polyglutamate. Finally, the electrophoretic behavior of polyglutamate backbone grafted with hydrophobic vitamin E (pGVE) nanogels according to the ionic strength was investigated using the recently proposed slope plot approach. It was deduced that the pGVE nanogels behave electrophoretically as polyelectrolytes which is in good agreement with the high water content of the nanogels.
在这项工作中，使用泰勒分散分析和毛细管电泳来表征基于接枝有疏水性维生素 E 基团的聚谷氨酸链的聚合物药物传递纳米凝胶的大小和电荷。疏水性维生素 E 基团在水中自组装形成小的疏水性纳米区，可容纳小分子药物或治疗性蛋白质。泰勒分散分析非常适合于确定纳米材料的重均流体力学半径，并获取关于聚合物样品的尺寸多分散性的信息。有效电荷可以通过电泳迁移率和电泳模型（比较了三种不同的方法）使用电泳建模或通过毛细管电泳中的间接 UV 检测来确定。研究了维生素 E 疏水性对聚合物有效电荷的影响。与未修饰的聚谷氨酸相比，维生素 E 的存在导致聚合物有效电荷急剧下降。最后，使用最近提出的斜率图方法研究了根据离子强度的疏水性维生素 E（pGVE）纳米凝胶的聚谷氨酸主链的电泳行为。推断出 pGVE 纳米凝胶作为聚电解质电泳，这与纳米凝胶的高含水量非常吻合。
