光声流式细胞术中的光学透明化。
Optical clearing in photoacoustic flow cytometry.
作者信息
Menyaev Yulian A, Nedosekin Dmitry A, Sarimollaoglu Mustafa, Juratli Mazen A, Galanzha Ekaterina I, Tuchin Valery V, Zharov Vladimir P
机构信息
Phillips Classic Laser and Nanomedicine Laboratories, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, 4301 W. Markham St., Little Rock, AR 72205 USA.
Saratov State University, 83 Astrakhanskaya St., Saratov, 410012 Russia ; Institute of Precise Mechanics and Control of RAS, 28 Rabochaya St., Saratov, 410028 Russia ; Optoelectronics and Measurement Techniques Laboratory, University of Oulu, P.O. BOX 4500, 90014 Finland.
出版信息
Biomed Opt Express. 2013 Nov 27;4(12):3030-41. doi: 10.1364/BOE.4.003030. eCollection 2013.
Clinical applications of photoacoustic (PA) flow cytometry (PAFC) for detection of circulating tumor cells in deep blood vessels are hindered by laser beam scattering, that result in loss of PAFC sensitivity and resolution. We demonstrate biocompatible and rapid optical clearing (OC) of skin to minimize light scattering and thus, increase optical resolution and sensitivity of PAFC. OC effect was achieved in 20 min by sequent skin cleaning, microdermabrasion, and glycerol application enhanced by massage and sonophoresis. Using 0.8 mm mouse skin layer over a blood vessel in vitro phantom we demonstrated 1.6-fold decrease in laser spot blurring accompanied by 1.6-fold increase in PA signal amplitude from blood background. As a result, peak rate for B16F10 melanoma cells in blood flow increased 1.7-fold. By using OC we also demonstrated the feasibility of PA contrast improvement for human hand veins.
光声（PA）流式细胞术（PAFC）在检测深部血管中循环肿瘤细胞的临床应用受到激光束散射的阻碍，这会导致PAFC灵敏度和分辨率的损失。我们展示了对皮肤进行生物相容性快速光学清除（OC），以尽量减少光散射，从而提高PAFC的光学分辨率和灵敏度。通过依次进行皮肤清洁、微晶磨皮以及通过按摩和超声透入增强甘油涂抹，在20分钟内实现了OC效果。在体外模型中，在血管上方使用0.8毫米厚的小鼠皮肤层，我们证明激光光斑模糊减少了1.6倍，同时血液背景的PA信号幅度增加了1.6倍。结果，血流中B16F10黑色素瘤细胞的峰值速率提高了1.7倍。通过使用OC，我们还证明了改善人手部静脉PA对比度的可行性。
Zotero 插件