Department of Clinical Pathophysiology, Tokyo Dental College, Chiba, Japan.
J Biomed Mater Res A. 2014 Apr;102(4):1180-6. doi: 10.1002/jbm.a.34765. Epub 2013 Aug 17.
The purpose of this study was to investigate osteogenesis using an artificial fusion protein (AFP) composed of modified bone morphogenetic protein 2 (BMP-2) with a titanium (Ti)-binding peptide (TBP) motif on a Ti surface in vivo. In the in vivostudy, 5-μm thick Ti was coated with electron cyclotron resonance sputtering on a porous carbon scaffold which was then dipped in one of three different mixtures of collagen gel: (1) collagen gel only, (2) collagen gel with TBP, and (3) collagen gel with the AFP between BMP-2 and the TBP motif (AFP-TBP-BMP-2). These scaffolds were then implanted into rat abdominal muscles and were studied histologically at various times and the expression of several bone-related protein messenger RNAs (mRNAs) was also analyzed. The Ti-coated scaffold of the collagen gel with AFP-TBP-BMP-2 produced cartilage in the muscle and the expression of alkaline phosphatase, bone sialoprotein, and runt-related gene 2 mRNAs was significantly increased. These results suggest that the scaffold of the collagen gel with AFP-TBP-BMP-2 accelerates osteogenesis in vivo.
本研究旨在探讨在钛表面上的人工融合蛋白（AFP）对骨生成的作用，该 AFP 由经修饰的骨形态发生蛋白 2（BMP-2）与钛结合肽（TBP）基序组成。在体内研究中，使用电子回旋共振溅射在多孔碳支架上涂覆 5μm 厚的钛，然后将其浸入三种不同的胶原凝胶混合物之一中：（1）仅胶原凝胶，（2）含 TBP 的胶原凝胶，和（3）在 BMP-2 和 TBP 基序之间的 AFP-TBP-BMP-2 胶原凝胶。然后将这些支架植入大鼠的腹肌中，并在不同时间进行组织学研究，同时还分析了几种与骨相关的蛋白信使 RNA（mRNA）的表达。含 AFP-TBP-BMP-2 的胶原凝胶支架在肌肉中产生了软骨，碱性磷酸酶、骨涎蛋白和 runt 相关基因 2 mRNA 的表达显著增加。这些结果表明，含 AFP-TBP-BMP-2 的胶原凝胶支架可加速体内成骨作用。
