Howell S B, Dean J H, Law L W
Int J Cancer. 1975 Jan 15;15(1):152-69. doi: 10.1002/ijc.2910150118.
Four assays of tumor-specific cell-mediated immunity were compared during growth of a syngeneic Simian-virus-40-induced tumor, mKSA. Major differences were found in immunity detected by the tumor neutralization test (the Winn test), direct tumor challenge, the microcytotoxicity assay and the -51chromium-release lymphocytotoxicity assay. Progressive growth of the neoplasm followed by loss of immunity (the eclipse phenomenon) was documented with the Winn test. It was established that this eclipse phenomenon represented a lesion in the T-cell system of tumor-bearing hosts. This lesion was found to be specific and unrelated to anatomic tumor location. The ability to produce graft-versus-host reactions, and the ability to respond to mitogens, were found to be generally intact in tumor-bearing animals. Cells capable of recognizing mKSA tumor antigens and reconstituting an immune response following surgical removal of tumor or upon transfer to normal mice were found in the spleens of mice bearing advanced tumors. No suppressor cells that might account for the eclipse phenomenon could be demonstrated. Tumor-bearer serum did not block neutralizing activity of immune spleen cells in the Winn test, and immune cells were capable of neutralizing tumor even in tumor-bearing hosts. The possibility that the lesion is intrinsic to T-cell precursors of effector cells is discussed.
在同基因猿猴病毒40诱导的肿瘤mKSA生长过程中，对四种肿瘤特异性细胞介导免疫测定法进行了比较。在通过肿瘤中和试验（温氏试验）、直接肿瘤攻击、微量细胞毒性测定法和51铬释放淋巴细胞毒性测定法检测到的免疫方面发现了主要差异。温氏试验记录了肿瘤的进行性生长以及随后免疫丧失（日食现象）。已确定这种日食现象代表荷瘤宿主T细胞系统中的一种病变。发现这种病变是特异性的，与肿瘤的解剖位置无关。发现荷瘤动物产生移植物抗宿主反应的能力和对有丝分裂原的反应能力通常是完整的。在患有晚期肿瘤的小鼠脾脏中发现了能够识别mKSA肿瘤抗原并在手术切除肿瘤后或转移到正常小鼠后重建免疫反应的细胞。未发现可能解释日食现象的抑制细胞。在温氏试验中，荷瘤动物血清不会阻断免疫脾细胞的中和活性，并且免疫细胞即使在荷瘤宿主中也能够中和肿瘤。讨论了该病变是效应细胞T细胞前体固有的可能性。
