Mustafaev M I, Babakhin A A, Popov A N, Litvinov I S, Merkushov A V, Gushchin I S
Mol Biol (Mosk). 1990 Mar-Apr;24(2):358-69.
Immunogenicity of soluble protein antigens in the complexes with synthetic polyions may be regarded as depending both on the nature of polymer carrier and the structure of the protein-polyelectrolyte complex. The immunogenicity of stable soluble complexes of ovalbumin (OA) with polycation - quaternized poly-4-vinylpyridine (C-1) and copolymer of acrylic acid and 2-methyl-5-vinylpyridine (C-2) have been evaluated. Immunization of mice by C-1 have induced a vigorous formation of the anti-OA IgG antibodies and IgE homocytotropic antibodies, while immunogenicity of OA in C-2 was comparable with that of OA alone. The analysis of the structural-chemical features of the complexes investigated has shown that enhanced immunogenicity of C-1 may be due to (1) the non-homogeneous distribution of protein globulae among polycation macromolecules and to (2) the formation of complex with an asymmetrical structure, to (3) the high ability of C-1 to adsorb on a surface of the lymphoid cells and to induce a formation of intercellular aggregates. An enhancing of a stability and a size of C-2 in the presence of Cu2+ shows no influence on a immunogenicity of OA. An immunogenicity of both types of complexes does not depend upon the access of determinants of OA to antibodies so far as it has been shown that complex formation in both cases are not accompanied by an alteration of antigenicity and allergenicity of OA.
可溶性蛋白质抗原与合成聚离子形成的复合物的免疫原性，可能被认为既取决于聚合物载体的性质，也取决于蛋白质 - 聚电解质复合物的结构。已对卵清蛋白（OA）与聚阳离子——季铵化聚-4-乙烯基吡啶（C-1）以及丙烯酸与2-甲基-5-乙烯基吡啶的共聚物（C-2）形成的稳定可溶性复合物的免疫原性进行了评估。用C-1免疫小鼠可诱导抗OA IgG抗体和IgE亲细胞性抗体的强烈形成，而OA在C-2中的免疫原性与单独的OA相当。对所研究复合物的结构化学特征分析表明，C-1免疫原性增强可能是由于：（1）蛋白质球体在聚阳离子大分子间的非均匀分布；（2）形成具有不对称结构的复合物；（3）C-1在淋巴细胞表面的高吸附能力以及诱导细胞间聚集体的形成。在Cu2+存在下C-2稳定性和尺寸的增加对OA的免疫原性没有影响。这两种复合物的免疫原性均不取决于OA的决定簇与抗体的接触，因为已表明在这两种情况下复合物的形成均未伴随OA抗原性和变应原性的改变。
