Kabanov V A, Mustafaev M I, Belova V V, Evdakov V P
Mol Biol (Mosk). 1978 Nov-Dec;12(6):1264-73.
Interaction of bonive serum albumin with copolymers 2-methyl-5-vinylpyridine, acrylic and methacrylic acids in different proportions in aqueous solutions was studied. It was shown, that in a wide range of ration of the components soluble cooperative complexes were formed. Ability of amphotheric copolymers to form complexes with bovine serum albumin depend both on the composition of the copolymers and on the pH of the medium. The non-polar interactions of the protein globules with hydrocarbonic 2-methyl-5-vinylpyridine chain fragments of the polyampholyte play an important role in the association of similarly charged particals. It was shown that besides this effect of chain fragments of 2-methyl-5-vinylpyridine a significant contribution in the association is made by non-polar interactions, created by methyl groups in copolymer 2-methyl-5-vinylpyridine and methacrylic acid. The association mechanism and structure of the formed complexes was studied. It was shown that complex formation is accompanied by non-equal distribution of the chain copolymer between the protein globules and appearance of asymmetric particles of the complex. A correlation between the ability of polyampholytes to bind protein and their physiological action was found.
研究了牛血清白蛋白与2-甲基-5-乙烯基吡啶、丙烯酸和甲基丙烯酸按不同比例组成的共聚物在水溶液中的相互作用。结果表明，在较宽的组分比例范围内形成了可溶性协同配合物。两性共聚物与牛血清白蛋白形成配合物的能力既取决于共聚物的组成，也取决于介质的pH值。蛋白质球粒与聚两性电解质的烃类2-甲基-5-乙烯基吡啶链片段的非极性相互作用在带相同电荷粒子的缔合中起重要作用。结果表明，除了2-甲基-5-乙烯基吡啶链片段的这种作用外，由2-甲基-5-乙烯基吡啶和甲基丙烯酸共聚物中的甲基产生的非极性相互作用对缔合也有显著贡献。研究了形成的配合物的缔合机理和结构。结果表明，配合物的形成伴随着链状共聚物在蛋白质球粒之间的不均匀分布以及配合物不对称颗粒的出现。发现聚两性电解质结合蛋白质的能力与其生理作用之间存在相关性。
