Jin Yu, Zhang Rong-Fang, Xie Zhi-Ping, Gao Han-Chun, Yan Kun-Long, Yuan Xin-Hui, Song Jing-Rong, Hou Yun-De, Duan Zhao-Jun
Nanjing Children' s Hospital, Nanjing Medical University, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2012 Dec;26(6):409-11.
To investigate the prevalence and clinical characterization of HCoV-NL63 (NL63) in children with acute respiratory tract infections (ARTIs) in Lanzhou with other respiratory viruses. The prevalence of HBoV1 in ALRTI was obviously city,China.
From November 2006 to October 2009,1169 nasopharyngeal aspirates (NPA) were collected from children under 14 years old with ARTIs. Samples were screened for NL63 using a reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. Demography and clinical information were recorded.
NL63 was detected in 35 (2.99%) of the 1169 children. The peak of the positive rate were in August, September 2007, July, August 2008 (23.53%,17.65%, 50%, 33.33% separately). There are no NL63 positive samples was detected in December, 2007 to February 2009. 25 (25/35, 71.43%) were co-infected with other respiratory viruses, and human rhinovirus (HRV) were the most common additional respiratory virus. No significant differences of infective rate of NL63 was found between < or = 3 years age group and > 3 years age group. Bronchiolitis and pneumonia were the most frequent diagnoses in NL63 positive patients and the major symptoms were fever and cough in our study. Between the monoinfection group and the coinfection group of NL63-positive patients, no differences were found in symptoms and clinical diagnoses except symptoms of gastrointestinal.
HCoV-NL63 is an important pathogen of acute respiratory tract infection in children in Lanzhou city. The peak of HCoV-NL63 infections was in summer. There were annual differences in the prevalence of HCoV-NL63. HCoV-NL63 infections existed a high rate of mixed infection, and mixed infection does not increase the severity of the disease.
调查中国兰州急性呼吸道感染（ARTIs）儿童中HCoV-NL63（NL63）的流行情况及其与其他呼吸道病毒的临床特征。人博卡病毒1型（HBoV1）在急性下呼吸道感染（ALRTI）中的流行情况在中国明显存在城市差异。
2006年11月至2009年10月，收集1169例14岁以下ARTIs儿童的鼻咽抽吸物（NPA）。采用逆转录聚合酶链反应（RT-PCR）和测序对样本进行NL63筛查。记录人口统计学和临床信息。
1169例儿童中35例（2.99%）检测到NL63。阳性率高峰出现在2007年8月、9月，2008年7月、8月（分别为23.53%、17.65%、50%、33.33%）。2007年12月至2009年2月未检测到NL63阳性样本。25例（25/35，71.43%）与其他呼吸道病毒合并感染，人鼻病毒（HRV）是最常见的合并呼吸道病毒。NL63感染率在≤3岁年龄组和＞3岁年龄组之间无显著差异。细支气管炎和肺炎是NL63阳性患者最常见的诊断，本研究中的主要症状为发热和咳嗽。NL63阳性患者的单感染组和合并感染组之间，除胃肠道症状外，症状和临床诊断无差异。
HCoV-NL63是兰州市儿童急性呼吸道感染的重要病原体。HCoV-NL63感染高峰在夏季。HCoV-NL63的流行存在年度差异。HCoV-NL63感染存在较高的混合感染率，且混合感染不会增加疾病的严重程度。
