[天麻中天麻素含量的提取与检测方法研究]
[Research the extraction and detection method of gastrodin content in Gastrodia elata].
作者信息
Xu Ding-Qiao, Zhou Jian-Jun, Liu Yi-Hong
机构信息
School of Biological Science and Engineering, Shaanxi University of Technology/The Center of Gastrodia elata B1. and Dogwood Engineering in Shaanxi, Hanzhong 723000, China.
出版信息
Zhong Yao Cai. 2012 Nov;35(11):1799-804.
OBJECTIVE
To improve an extraction and determination method for gastrodin in Gastrodia elata.
METHODS
On the basis of single factor experiment, used the method of hot water reflux extraction and applied the response surface methodology to determine the best conditions for extracting gastrodin and using HPLC test.
RESULTS
Under the boiling water condition, pure water as solvent, the solid-liquid ratio was 1:37,extraction 2 times and extraction time 126 min per times,the content of gastrodin was 0.377%. Comparison with the 2010 edition of the China pharmacopoeia of gastrodin extracted in Gastrodia elata, the result showed the gastrodin content was increased by 2. 12 times.
CONCLUSION
This extraction method of gastrodin dissolution rate is high and can reflect the real content of gastrodin of Gastrodia elata.
目的
改进天麻中天麻素的提取与测定方法。
方法
在单因素实验的基础上，采用热水回流提取法，并应用响应面法确定天麻素提取的最佳条件，采用高效液相色谱法进行检测。
结果
在沸水条件下，以纯水为溶剂，固液比为1:37，提取2次，每次提取时间126分钟，天麻素含量为0.377%。与《中国药典》2010年版中天麻提取的天麻素含量比较，结果表明天麻素含量提高了2.12倍。
结论
该天麻素提取方法溶出率高，能反映天麻中天麻素的实际含量。
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