Ong Eng Shi, Heng Ming Yuan, Tan Swee Ngin, Hong Yong Jean Wan, Koh Huijing, Teo Chin Chye, Hew Choy Sin
Department of Community, Occupational and Family Medicine, National University of Singapore, Singapore.
J Sep Sci. 2007 Aug;30(13):2130-7. doi: 10.1002/jssc.200700002.
Pressurized liquid extraction (PLE) at room temperature with a laboratory-assembled system was applied for the extraction of gastrodin (GA) and vanillyl alcohol (VA) in Gastrodia elata Blume. The proposed system setup for this current work was simpler as no heating and backpressure regulator was required. Extraction with PLE was carried out dynamically at a flow rate of 1.5 mL/min, at room temperature, under an applied pressure of 10-20 bars with an extraction time of 40-50 min. The extraction efficiencies of the proposed method using 20% aqueous ethanol were compared with heating under reflux using organic solvents such as methanol and ethanol/water (20:80) for different batches of medicinal plant materials. For the determination of GA and VA in G. elata Blume, the extraction efficiencies of PLE at room temperature were observed to be comparable with heating under reflux. The method precision was found to vary from 1.6 to 8.6% (RSD, n = 6) on different days. The marker compounds present in the various medicinal plant extracts were determined by gradient elution HPLC and HPLC/MS/MS. Our work demonstrated the possibility of implementation of PLE at room temperature and the advantages of minimizing the use of organic solvents in the extraction process.
采用实验室组装的系统在室温下进行加压液体萃取（PLE），用于提取天麻中的天麻素（GA）和香草醇（VA）。当前这项工作所提出的系统设置更为简单，因为不需要加热和背压调节器。PLE萃取在室温下以1.5 mL/min的流速动态进行，施加压力为10 - 20巴，萃取时间为40 - 50分钟。使用20%乙醇水溶液的该方法的萃取效率与使用甲醇和乙醇/水（20:80）等有机溶剂回流加热的方法针对不同批次药用植物材料进行了比较。对于天麻中GA和VA的测定，观察到室温下PLE的萃取效率与回流加热相当。该方法的精密度在不同日期为1.6%至8.6%（相对标准偏差，n = 6）。通过梯度洗脱HPLC和HPLC/MS/MS测定各种药用植物提取物中的标记化合物。我们的工作证明了在室温下实施PLE的可能性以及在萃取过程中尽量减少有机溶剂使用的优势。
