Van Kruiningen Herbert J, Heishima Mizuki, Kerr Kirklyn M, Garmendia Antonio E, Helal Zeinab, Smyth Joan A
Connecticut Veterinary Medical Diagnostic Laboratory, Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT.
J Vet Diagn Invest. 2019 Sep;31(5):732-736. doi: 10.1177/1040638719863102. Epub 2019 Jul 10.
A 5-mo-old Bassett Hound-Labrador Retriever cross was autopsied following a bout of lethargy, inappetence, and bleeding gums. Mucous membranes were white, and the small intestine was blue-black; the colon contained black feces. The spleen was swollen, and multiple lymph nodes were enlarged and hemorrhagic. Microscopically, the small intestine had focal crypt cell necrosis and circumferential transmural vasculitis, the latter the cause of infarction and the blue-black coloration. Lymphocytes were necrotic in spleen and lymph nodes, and erythrophagocytosis was present in some nodes. Vasculitis was present in brain, meninges, lung, liver, and kidneys. Electron microscopy revealed aggregates of 15-18 nm round viral particles in damaged crypt cells and in the endothelium of small blood vessels. Electron-dense intracytoplasmic inclusions consisting of paracrystalline-arrayed virus were demonstrated in macrophages in medullary lymph node sinuses. These virions were identified as circovirus, which was confirmed by real-time PCR and sequencing.
一只5月龄的巴吉度猎犬与拉布拉多犬的杂交幼犬，在出现一阵嗜睡、食欲不振和牙龈出血后进行了尸检。黏膜呈白色，小肠呈蓝黑色；结肠内有黑色粪便。脾脏肿大，多个淋巴结肿大且有出血。显微镜下，小肠有局灶性隐窝细胞坏死和全层透壁性血管炎，后者是梗死和蓝黑色变色的原因。脾脏和淋巴结中的淋巴细胞坏死，部分淋巴结中有红细胞吞噬现象。脑、脑膜、肺、肝和肾中均存在血管炎。电子显微镜检查显示，受损隐窝细胞和小血管内皮中有15 - 18纳米的圆形病毒颗粒聚集。在髓质淋巴结窦的巨噬细胞中发现了由呈准晶体排列的病毒组成的电子致密胞质内包涵体。这些病毒粒子被鉴定为圆环病毒，通过实时PCR和测序得以证实。
