Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Goethe University, Frankfurt am Main, Germany.
Curr Opin Struct Biol. 2013 Jun;23(3):374-80. doi: 10.1016/j.sbi.2013.03.012. Epub 2013 Apr 27.
Cell-free protein production opens new perspectives for the direct manipulation of expression compartments in combination with reduced complexity of physiological requirements. The technology is therefore in particular suitable for the general synthesis of difficult proteins including toxins and membrane proteins as well as for the analysis of their functional folding in artificial environments. A further key application of cell-free expression is the fast and economic labeling of proteins for structural and functional applications. Two extract sources, wheat embryos and Escherichia coli cells, are currently employed for the preparative scale cell-free production of proteins. Recent achievements in structural characterization include cell-free synthesized membrane proteins and even larger protein assemblies may become feasible.
无细胞蛋白质生产为直接操作表达隔室开辟了新的前景，同时降低了对生理需求的复杂性。因此，该技术特别适用于包括毒素和膜蛋白在内的困难蛋白质的一般合成，以及在人工环境中分析它们的功能折叠。无细胞表达的另一个关键应用是快速、经济地标记蛋白质，用于结构和功能应用。目前，小麦胚和大肠杆菌细胞这两种提取物来源被用于大规模制备无细胞蛋白质生产。在结构特征描述方面的最新成果包括无细胞合成的膜蛋白，甚至更大的蛋白质组装也可能成为可行的。
