College of Pharmacy, Harbin Medical University, Harbin 150086, China.
Int J Biol Macromol. 2013 Aug;59:290-4. doi: 10.1016/j.ijbiomac.2013.04.067. Epub 2013 Apr 26.
In this study, effects of several experimental parameters, including ultrasonic time, ratio of water to material, extraction temperature and ultrasonic power on the extraction yield of polysaccharides from Acanthopanax senticosus stem (ASS) were investigated by single factor experiment and an orthogonal test design (L9(3)(4)) was used to optimize the ultrasound extraction conditions. The polysaccharides from Acanthopanax senticosus stem (ASSP) and fruit (ASFP) were further fractionated by stepwise ethanol precipitation and the anti-oxidation activities of those fractions were evaluated by hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Under the optimal conditions (ultrasonic time 75min, ratio of water to material 50ml/g, extraction temperature 80°C and ultrasonic power 100W), the yield was 10.9mg/g. All fractions of ASP possessed considerable antioxidant activity. The results indicated that the ultrasound extraction was a very useful method for the extraction of ASP and the polysaccharides could be explored as a potential antioxidant agent for use in medicine or functional food.
在这项研究中，通过单因素实验和正交试验设计（L9(3)(4)）考察了几种实验参数对刺五加茎（ASS）多糖提取率的影响，包括超声时间、水料比、提取温度和超声功率。进一步采用分步乙醇沉淀法对刺五加茎（ASSP）和果实（ASFP）多糖进行分级，并通过羟基、超氧阴离子和 1,1-二苯基-2-苦基肼（DPPH）自由基清除实验评价这些级分的抗氧化活性。在最优条件（超声时间 75min、水料比 50ml/g、提取温度 80°C 和超声功率 100W）下，多糖得率为 10.9mg/g。ASSP 的所有级分均具有相当的抗氧化活性。结果表明，超声提取是提取 ASP 的一种非常有用的方法，多糖可作为一种有潜力的抗氧化剂，用于医药或功能性食品。
