Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
Acta Biomater. 2013 Aug;9(8):7874-84. doi: 10.1016/j.actbio.2013.04.035. Epub 2013 Apr 27.
Mesenchymal progenitor cells (MPCs) represent an attractive cell population for bone tissue engineering. Their special immunological characteristics suggest that MPCs may be used in allogenic applications. The objective of this study was to compare the regenerative potential of autologous vs. allogenic MPCs in an ovine critical size segmental defect model. Ovine MPCs were isolated from bone marrow aspirates, expanded and cultured with osteogenic medium for 2weeks before implantation. Autologous and allogenic transplantation was performed using the cell-seeded scaffolds and unloaded scaffolds, while the application of autologous bone grafts served as a control group (n=6). Bone healing was assessed 12weeks after surgery by radiology, microcomputed tomography, biomechanical testing and histology. Radiology, biomechanical testing and histology revealed no significant differences in bone formation between the autologous and allogenic groups. Both cell groups showed more bone formation than the scaffold alone, whereas the biomechanical data showed no significant differences between the cell groups and the unloaded scaffolds. The results of the study suggest that scaffold-based bone tissue engineering using allogenic cells offers the potential for an off-the-shelf product. Thus the results of this study serve as an important baseline for translation of the assessed concepts into clinical applications.
间充质祖细胞（MPCs）是一种很有吸引力的用于骨组织工程的细胞群体。其特殊的免疫学特性表明 MPCs 可能用于同种异体应用。本研究的目的是比较自体与同种异体 MPC 在绵羊临界尺寸节段性缺损模型中的再生潜力。从骨髓抽吸物中分离出绵羊 MPC，并用成骨培养基培养 2 周，然后进行植入。使用细胞接种支架和未加载支架进行自体和同种异体移植，而自体骨移植物的应用作为对照组（n=6）。手术后 12 周通过放射学、微计算机断层扫描、生物力学测试和组织学评估骨愈合情况。放射学、生物力学测试和组织学显示，自体和同种异体组之间的骨形成没有显著差异。两个细胞组的骨形成均多于单独支架，而生物力学数据显示细胞组与未加载支架之间没有显著差异。研究结果表明，使用同种异体细胞的基于支架的骨组织工程为现成产品提供了可能性。因此，本研究的结果为将评估的概念转化为临床应用提供了重要的基础。
