在经过验证的临床前动物模型中的支架-细胞骨工程：前体细胞与分化细胞来源
Scaffold-cell bone engineering in a validated preclinical animal model: precursors vs differentiated cell source.
作者信息
Berner A, Henkel J, Woodruff M A, Saifzadeh S, Kirby G, Zaiss S, Gohlke J, Reichert J C, Nerlich M, Schuetz M A, Hutmacher D W
机构信息
Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
Department of Trauma Surgery, University of Regensburg, Germany.
出版信息
J Tissue Eng Regen Med. 2017 Jul;11(7):2081-2089. doi: 10.1002/term.2104. Epub 2015 Dec 9.
The properties of osteoblasts (OBs) isolated from the axial skeleton (tOBs) differ from OBs of the orofacial skeleton (mOBs) due to the different embryological origins of the bones. The aim of the study was to assess and compare the regenerative potential of allogenic bone marrow-derived mesenchymal progenitor cells with allogenic tOBs and allogenic mOBs in combination with a mPCL-TCP scaffold in critical-sized segmental bone defects in sheep tibiae. After 6 months, the tibiae were explanted and underwent biomechanical testing, micro-computed tomography (microCT) and histological and immunohistochemical analyses. Allogenic MPCs demonstrated a trend towards a better outcome in biomechanical testing and the mean values of newly formed bone. Biomechanical, microCT and histological analysis showed no significant differences in the bone regeneration potential of tOBs and mOBs in our in vitro study, as well as in the bone regeneration potential of different cell types in vivo. Copyright © 2015 John Wiley & Sons, Ltd.
由于骨骼的胚胎起源不同，从轴骨骼分离出的成骨细胞（tOBs）的特性与口面部骨骼的成骨细胞（mOBs）不同。本研究的目的是评估和比较同种异体骨髓来源的间充质祖细胞与同种异体tOBs和同种异体mOBs，并结合mPCL-TCP支架，在绵羊胫骨临界尺寸节段性骨缺损中的再生潜力。6个月后，取出胫骨并进行生物力学测试、显微计算机断层扫描（microCT）以及组织学和免疫组织化学分析。同种异体间充质祖细胞在生物力学测试和新形成骨的平均值方面显示出更好结果的趋势。生物力学、microCT和组织学分析表明，在我们的体外研究中，tOBs和mOBs的骨再生潜力以及体内不同细胞类型的骨再生潜力没有显著差异。版权所有© 2015 John Wiley & Sons, Ltd.
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