Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada.
FEBS Lett. 2013 Jun 5;587(11):1687-92. doi: 10.1016/j.febslet.2013.04.019. Epub 2013 Apr 27.
The purpose of this study was to examine the effects of sarcolipin (SLN) on sarco(endo) plasmic reticulum Ca(2+)-ATPase (SERCA pump) energetics in vivo and resting skeletal muscle metabolic rate. Using SLN knockout (Sln(-/-)) mice we show that SLN ablation increases the transport stoichiometry of SERCA pumps (Ca(2+) uptake/Ca(2+)-ATPase activity) and decreases the relative contribution of SERCA pumps to resting oxygen consumption (VO2) in soleus without affecting soleus or whole body VO2. These data suggest that the lower energy requirements for Ca(2+) cycling in resting skeletal muscle of Sln(-/-) mice do not impact significantly either skeletal muscle or whole body metabolic rate.
本研究旨在探讨肌联蛋白 (SLN) 对肌浆网（endo）钙转运 ATP 酶（SERCA 泵）能量代谢和骨骼肌代谢率的影响。利用 SLN 敲除（Sln(-/-)）小鼠，我们发现 SLN 缺失增加了 SERCA 泵的转运化学计量（Ca(2+)摄取/Ca(2+)-ATP 酶活性），降低了 SERCA 泵对比目鱼肌静息耗氧量（VO2）的相对贡献，而不影响比目鱼肌或全身 VO2。这些数据表明，SLN(-/-) 小鼠静息骨骼肌中 Ca(2+)循环的能量需求降低，对骨骼肌或全身代谢率的影响并不显著。
