Zhao Chuanqi, Qu Xiaogang
Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Methods. 2013 Nov;64(1):52-8. doi: 10.1016/j.ymeth.2013.04.017. Epub 2013 Apr 27.
Guanine-rich nucleic acids are known to form four-stranded G-quadruplex structures which are attracting increasing attention in diverse areas such as biology, medicinal chemistry, supramolecular chemistry and nanotechnology. To date, the handling media for DNA has largely been limited to an aqueous phase. Since many chemical reactions and devices are required to be performed under strictly anhydrous conditions, even at high temperature, it is meaningful but challenging to conduct G-quadruplex DNA in water-free medium. Recently, deep eutectic solvent (DES), a related material to ionic liquids (ILs) was considered as a new class of anhydrous media for DNA. This review highlights the stability, structure, folding dynamics and thermodynamics of G-quadruplex in DES. Spectroscopic methodologies, like circular dichroism, UV and fluorescence, are mainly employed in these studies.
富含鸟嘌呤的核酸已知会形成四链G-四链体结构，这种结构在生物学、药物化学、超分子化学和纳米技术等不同领域正受到越来越多的关注。迄今为止，DNA的处理介质在很大程度上局限于水相。由于许多化学反应和装置需要在严格无水的条件下进行，甚至是在高温下，因此在无水介质中处理G-四链体DNA既具有意义又具有挑战性。最近，深共熔溶剂（DES），一种与离子液体（ILs）相关的材料，被认为是用于DNA的一类新型无水介质。本综述重点介绍了G-四链体在DES中的稳定性、结构、折叠动力学和热力学。这些研究主要采用了光谱学方法，如圆二色性、紫外和荧光。
