Tateishi-Karimata Hisae, Sugimoto Naoki
Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojimaminamimachi, Kobe 650-0047, Japan.
Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojimaminamimachi, Kobe 650-0047, Japan Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojimaminamimachi, Kobe 650-0047, Japan
Nucleic Acids Res. 2014 Aug;42(14):8831-44. doi: 10.1093/nar/gku499. Epub 2014 Jul 10.
Nucleic acids have become a powerful tool in nanotechnology because of their conformational polymorphism. However, lack of a medium in which nucleic acid structures exhibit long-term stability has been a bottleneck. Ionic liquids (ILs) are potential solvents in the nanotechnology field. Hydrated ILs, such as choline dihydrogen phosphate (choline dhp) and deep eutectic solvent (DES) prepared from choline chloride and urea, are 'green' solvents that ensure long-term stability of biomolecules. An understanding of the behaviour of nucleic acids in hydrated ILs is necessary for developing DNA materials. We here review current knowledge about the structures and stabilities of nucleic acids in choline dhp and DES. Interestingly, in choline dhp, A-T base pairs are more stable than G-C base pairs, the reverse of the situation in buffered NaCl solution. Moreover, DNA triplex formation is markedly stabilized in hydrated ILs compared with aqueous solution. In choline dhp, the stability of Hoogsteen base pairs is comparable to that of Watson-Crick base pairs. Moreover, the parallel form of the G-quadruplex is stabilized in DES compared with aqueous solution. The behaviours of various DNA molecules in ILs detailed here should be useful for designing oligonucleotides for the development of nanomaterials and nanodevices.
由于核酸具有构象多态性，它们已成为纳米技术中的一种强大工具。然而，缺乏一种能使核酸结构表现出长期稳定性的介质一直是个瓶颈。离子液体（ILs）是纳米技术领域中潜在的溶剂。水合离子液体，如磷酸二氢胆碱（胆碱dhp）以及由氯化胆碱和尿素制备的低共熔溶剂（DES），都是能确保生物分子长期稳定性的“绿色”溶剂。了解核酸在水合离子液体中的行为对于开发DNA材料是必要的。我们在此综述了关于核酸在胆碱dhp和DES中的结构与稳定性的现有知识。有趣的是，在胆碱dhp中，A - T碱基对比G - C碱基对更稳定，这与在缓冲NaCl溶液中的情况相反。此外，与水溶液相比，DNA三链体的形成在水合离子液体中显著稳定。在胆碱dhp中，Hoogsteen碱基对的稳定性与Watson - Crick碱基对相当。此外，与水溶液相比，G - 四链体的平行形式在DES中得到稳定。这里详细介绍的各种DNA分子在离子液体中的行为对于设计用于开发纳米材料和纳米器件的寡核苷酸应该是有用的。
