Tawfeeq Mohammad Monir, Miura Saori, Horiuchi Noriyuki, Kobayashi Yoshiyasu, Furuoka Hidefumi, Inokuma Hisashi
Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
J Vet Med Sci. 2013;75(9):1167-72. doi: 10.1292/jvms.12-0572. Epub 2013 Apr 26.
This study evaluated the clinical usefulness of serum thymidine kinase (TK) activity for diagnosing bovine leukosis cases for which clinical diagnosis was difficult ('BL with difficult diagnosis'). Median TK activity values in 24 'BL with difficult diagnosis' and 36 cattle for which BL was clinically confirmed by cytology findings of enlarged superficial lymph nodes ('clinically confirmed BL') were 36.8 and 39.4 U/l, respectively (no significant difference). The percentage with positive TK activity (> 5.4 U/l) was also similar in both groups (83.3% for 'BL with difficult diagnosis' and 97.2% for 'clinically confirmed BL'). TK activity was significantly higher in cows with 'BL with difficult diagnosis' compared to those with other tumors (N = 13) and those with inflammatory diseases (N = 14). Maximum TK activity in cows with other tumors and inflammatory diseases was not high (< 10 U/l). Median TK activities in cows with other tumors and those with inflammatory diseases were 1.8 and 1.4 IU/l, respectively. Positive TK activity was found in a significantly higher percentage of cows with 'BL with difficult diagnosis' (83.3%) relative to the percentages of cows with other tumors (15.3%) and inflammatory diseases (21.4%). Thus, TK activity is an appropriate marker for detecting BL onset in cows with 'BL with difficult diagnosis' as well as 'clinically confirmed BL' group. While the specificity of TK activity required for BL diagnosis is not clear, simultaneous evaluation of serum lactate dehydrogenase activity may assist in the differential diagnoses of other tumors and inflammatory diseases from BL.
本研究评估了血清胸苷激酶（TK）活性在诊断临床诊断困难的牛白血病病例（“诊断困难的牛白血病”）中的临床实用性。24例“诊断困难的牛白血病”病例和36例经浅表淋巴结肿大的细胞学检查临床确诊为牛白血病的牛（“临床确诊的牛白血病”）的TK活性中值分别为36.8和39.4 U/l（无显著差异）。两组中TK活性阳性（>5.4 U/l）的百分比也相似（“诊断困难的牛白血病”组为83.3%，“临床确诊的牛白血病”组为97.2%）。与患有其他肿瘤（N = 13）和炎症性疾病（N = 14）的奶牛相比，“诊断困难的牛白血病”奶牛的TK活性显著更高。患有其他肿瘤和炎症性疾病的奶牛的最大TK活性不高（<10 U/l）。患有其他肿瘤和炎症性疾病的奶牛的TK活性中值分别为1.8和1.4 IU/l。与患有其他肿瘤（15.3%）和炎症性疾病（21.4%）的奶牛相比，“诊断困难的牛白血病”奶牛中TK活性阳性的百分比显著更高（83.3%）。因此，TK活性是检测“诊断困难的牛白血病”奶牛以及“临床确诊的牛白血病”组中牛白血病发病的合适标志物。虽然牛白血病诊断所需的TK活性特异性尚不清楚，但同时评估血清乳酸脱氢酶活性可能有助于从牛白血病中鉴别诊断其他肿瘤和炎症性疾病。
