Sakamoto Leo, Ohbayashi Tetsu, Matsumoto Kotaro, Kobayashi Yoshiyasu, Inokuma Hisashi
Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
J Vet Diagn Invest. 2009 Nov;21(6):871-4. doi: 10.1177/104063870902100619.
Serum thymidine kinase (TK) activity has recently been evaluated as a serum marker for human and canine hematopoietic neoplasms. The purpose of the current study was to establish the significance of serum TK activity in the diagnosis of bovine leukosis. The discrimination value for TK activity was set at 5.4 U/l based on the receiver operating characteristic curve. In the group of clinically healthy cows, only 2 out of 83 cows (2.4%) had serum TK activity above the discrimination value. In contrast, 19 out of 20 cows (95.0%) with bovine leukosis showed serum TK activity above the discrimination value, although only 7 of 79 (8.9%) cows diagnosed with diseases other than bovine leukosis showed elevated serum TK activity. Thymidine kinase activities of all Bovine leukemia virus-positive cows with or without lymphocytosis were below the discrimination value. Sensitivity and specificity of measuring serum TK activity as a diagnostic tool for bovine leukosis was 95.0% and 95.9%, respectively. Results indicate that serum TK activity may be a marker for bovine leukosis.
血清胸苷激酶（TK）活性最近已被评估为人类和犬类造血肿瘤的血清标志物。本研究的目的是确定血清TK活性在牛白血病诊断中的意义。根据受试者工作特征曲线，将TK活性的判别值设定为5.4 U/l。在临床健康奶牛组中，83头奶牛中只有2头（2.4%）血清TK活性高于判别值。相比之下，20头患牛白血病的奶牛中有19头（95.0%）血清TK活性高于判别值，而在79头被诊断为非牛白血病疾病的奶牛中，只有7头（8.9%）血清TK活性升高。所有无论有无淋巴细胞增多症的牛白血病病毒阳性奶牛的胸苷激酶活性均低于判别值。将测量血清TK活性作为牛白血病诊断工具的敏感性和特异性分别为95.0%和95.9%。结果表明，血清TK活性可能是牛白血病的一个标志物。
