Burke A P, Sobin L H, Shekitka K M, Avallone F A
Department of Gastrointestinal, Armed Forces Institute of Pathology, Washington, D.C.
Mod Pathol. 1990 May;3(3):357-60.
Forty-seven biopsies of gastric mucosa and Barrett esophagus from 32 patients were studied with the argyrophilic nucleolar organizer region method. Twenty-two biopsies were gastric and 25 esophageal. Four showed normal noninflamed mucosa, 14 reactive glandular changes, eight intestinal metaplasia without dysplasia, ten low grade dysplasia with intestinal metaplasia, and 11 high grade dysplasia. The mean number of nucleolar organizer regions was 14.9 for high grade dysplasia, 10.9 for low grade dysplasia, 8.5 for intestinal metaplasia without dysplasia, 6.7 for reactive changes, and 3.9 for normal mucosa. The difference between high grade dysplasia and the other groups was significant (P = 0.004). However, the difference between high and low grade dysplasia was not significant (P = 0.06), and there was an overlap between reactive and high grade dysplastic lesions. We conclude that although nucleolar organizer counts correlate with the degree of dysplasia, the technique is of limited practical use.
采用嗜银核仁组成区方法对32例患者的47份胃黏膜和巴雷特食管活检标本进行了研究。其中22份为胃活检标本，25份为食管活检标本。4份显示正常无炎症黏膜，14份有反应性腺体改变，8份为无发育异常的肠化生，10份为伴有肠化生的低级别发育异常，11份为高级别发育异常。高级别发育异常的核仁组成区平均数量为14.9，低级别发育异常为10.9，无发育异常的肠化生为8.5，反应性改变为6.7，正常黏膜为3.9。高级别发育异常与其他组之间的差异具有显著性（P = 0.004）。然而，高级别与低级别发育异常之间的差异不具有显著性（P = 0.06），并且反应性病变与高级别发育异常病变之间存在重叠。我们得出结论，虽然核仁组成区计数与发育异常程度相关，但该技术的实际应用价值有限。
