Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA.
J Biomed Opt. 2013 May;18(5):50502. doi: 10.1117/1.JBO.18.5.050502.
Miniature endoscopic probes offer a solution for deep brain imaging by overcoming the limited depth of intravital microscopy. We describe a small-diameter (350 μm) graded-index optical probe with a side-view design for in vivo cellular imaging of the mammalian brain. The side-view probe provides a unique view of the vertical network of neurons and penetrating blood vessels. At a given insertion site, the translational and rotational scanning of the probe provides access to a large tissue area (>1 mm(2)) across the cortex, hippocampus, thalamus, and hypothalamus.
微型内窥镜探头通过克服活体显微镜的有限深度，为深部脑成像提供了一种解决方案。我们描述了一种小直径（350μm）梯度折射率光探头，具有侧视设计，用于哺乳动物大脑的细胞在体成像。侧视探头提供了一个独特的垂直神经元网络和穿透血管的视图。在给定的插入位置，探头的平移和旋转扫描提供了对跨越皮层、海马体、丘脑和下丘脑的大片组织区域（>1mm²）的访问。
