Kim Jun Ki, Choi Jin Woo, Yun Seok H
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, Massachusetts 02114, USA ; WCU Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, South Korea ; Equal Contribution.
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, Boston, Massachusetts 02114, USA ; Currently with the Department of Dental Pharmacology and Institute of Biomaterials-Implant, School of Dentistry, Wonkwang University, Iksan, Chonbuk 570-749, South Korea ; Equal Contribution.
Biomed Opt Express. 2013 Nov 15;4(12):2846-54. doi: 10.1364/BOE.4.002846. eCollection 2013.
We demonstrate optical fine-needle imaging biopsy (FNIB), combining a fine needle (22 gauge) and a high-resolution side-view probe (350-μm diameter) for minimally invasive interrogation of brain tissue in situ. We apply this technique to examine pathogenesis in murine models of neurodegeneration, brain metastasis of melanoma, and arterial occlusion, respectively. The demonstrated ability to obtain cellular images in the deep brain without craniotomy may be useful in the longitudinal studies of brain diseases.
我们展示了光学细针成像活检（FNIB），它结合了一根细针（22号）和一个高分辨率侧视探头（直径350μm），用于对原位脑组织进行微创检查。我们分别应用该技术来研究神经退行性变、黑色素瘤脑转移和动脉闭塞的小鼠模型中的发病机制。所展示的在不进行开颅手术的情况下获取深部脑组织细胞图像的能力，可能对脑部疾病的纵向研究有用。
