Butterfield Jacqualine, Hong Weili, Mershon Leslie, Vershinin Michael
Department of Physics and Astronomy, University of Utah, USA.
J Vis Exp. 2013 Apr 22(74):50481. doi: 10.3791/50481.
High resolution microscope systems with optical traps allow for precise manipulation of various refractive objects, such as dielectric beads (1) or cellular organelles (2,3), as well as for high spatial and temporal resolution readout of their position relative to the center of the trap. The system described herein has one such "traditional" trap operating at 980 nm. It additionally provides a second optical trapping system that uses a commercially available holographic package to simultaneously create and manipulate complex trapping patterns in the field of view of the microscope (4,5) at a wavelength of 1,064 nm. The combination of the two systems allows for the manipulation of multiple refractive objects at the same time while simultaneously conducting high speed and high resolution measurements of motion and force production at nanometer and piconewton scale.
带有光阱的高分辨率显微镜系统能够精确操控各种折射物体，如介电珠（1）或细胞器（2,3），还能对其相对于阱中心的位置进行高空间和时间分辨率的读出。本文所述系统有一个在980纳米波长下运行的此类“传统”阱。它还提供了第二个光学捕获系统，该系统使用市售全息组件，在1064纳米波长下，于显微镜视场中同时创建和操控复杂的捕获图案（4,5）。这两个系统的组合使得能够同时操控多个折射物体，同时在纳米和皮牛顿尺度上对运动和力的产生进行高速和高分辨率测量。
