Department of Psychiatry and Behavioral Sciences, Center for Sleep Sciences, Beckman Center, Stanford University Palo Alto, CA, USA.
Front Neural Circuits. 2013 Apr 24;7:76. doi: 10.3389/fncir.2013.00076. eCollection 2013.
Recent advances in imaging tools are inspiring zebrafish researchers to tackle ever more ambitious questions in the neurosciences. Behaviorally fundamental conserved neural networks can now be potentially studied using zebrafish from a brain-wide scale to molecular resolution. In this perspective, we offer a roadmap by which a zebrafish researcher can navigate the course from collecting neural activities across the brain associated with a behavior, to unraveling molecular identities and testing the functional relevance of active neurons. In doing so, important insights will be gained as to how neural networks generate behaviors and assimilate changes in synaptic connectivity.
成像工具的最新进展激发了斑马鱼研究人员去解决神经科学中更具挑战性的问题。现在，从全脑尺度到分子分辨率，行为基础的保守神经网络都可以用斑马鱼来进行潜在研究。在此视角下，我们提供了一张路线图，让斑马鱼研究人员可以从记录与行为相关的大脑中的神经活动开始，到揭示分子身份并测试活跃神经元的功能相关性，从而驾驭整个过程。这样做将深入了解神经网络如何产生行为以及如何同化突触连接的变化。
