Chen Weijie, Xie Lv, Yu Fang, Li Yan, Chen Chen, Xie Wanqing, Huang Tingting, Zhang Yueman, Zhang Song, Li Peiying
Department of Anesthesiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine Shanghai Jiaotong University, 160 Pujian Rd, Shanghai, 200127, China.
Transl Stroke Res. 2021 Oct;12(5):695-710. doi: 10.1007/s12975-021-00907-3. Epub 2021 May 29.
Stroke is one of the world's leading causes of death and disability, posing enormous burden to the society. However, the pathogenesis and mechanisms that underlie brain injury and brain repair remain largely unknown. There's an unmet need of in-depth mechanistic research in this field. Zebrafish (Danio rerio) is a powerful tool in brain science research mainly due to its small size and transparent body, high genome synteny with human, and similar nervous system structures. It can be used to establish both hemorrhagic and ischemic stroke models easily and effectively through different ways. After the establishment of stroke model, research methods including behavioral test, in vivo imaging, and drug screening are available to explore mechanisms that underlie the brain injury and brain repair after stroke. This review focuses on the advantages and the feasibility of zebrafish stroke model, and will also introduce the key methods available for stroke studies in zebrafish, which may drive future mechanistic studies in the pursuit of discovering novel therapeutic targets for stroke patients.
中风是全球主要的死亡和致残原因之一，给社会带来了巨大负担。然而，脑损伤和脑修复的发病机制在很大程度上仍不清楚。该领域对深入的机制研究存在未满足的需求。斑马鱼（Danio rerio）是脑科学研究中的一种强大工具，主要是因为其体型小、身体透明、与人类基因组共线性高以及神经系统结构相似。通过不同方式，它可轻松有效地建立出血性和缺血性中风模型。中风模型建立后，可采用行为测试、体内成像和药物筛选等研究方法来探索中风后脑损伤和脑修复的机制。本综述重点介绍斑马鱼中风模型的优势和可行性，还将介绍斑马鱼中风研究可用的关键方法，这可能推动未来的机制研究，以寻找中风患者的新治疗靶点。
