Marchand F, Ahmed A M
Department of Mechanical Engineering, McGill University, Montreal, Quebec, Canada.
Spine (Phila Pa 1976). 1990 May;15(5):402-10. doi: 10.1097/00007632-199005000-00011.
The structure of the lumbar disc anulus fibrosus was investigated using a layer-by-layer peeling technique and microscopic examination of various cut surfaces. Anulus specimens from spines of two different age groups and from two levels, L2-3 and L4-5, were examined. The vertebra-disc-vertebra units were subjected to intentional controlled dehydration to enhance the visual contrast between the white opaque fiber bundles and the translucent ground substance. The variations of the anulus structure with circumferential and radial locations were studied. The following principal structural features were quantified: 1) the anulus, excluding the transition zone, consists of 15 to 25 distinct layers, depending on the circumferential location, the spine level, and the specimen age; 2) in any 20 degrees circumferential sector, nearly half of the layers terminate or originate, thereby causing local laminate irregularities; 3) there are two identifiable mechanisms of layer interruption at these irregularities; 4) the thickness of individual layers varies both circumferentially and radially and increases markedly with age; and 5) the number of fiber bundles over the total height of the disc varies from 20 to 62, with an average interbundle spacing of 0.22 mm.
采用逐层剥离技术并对不同切面进行显微镜检查，对腰椎间盘纤维环的结构进行了研究。对来自两个不同年龄组、两个节段（L2 - 3和L4 - 5）脊柱的纤维环标本进行了检查。对椎体 - 椎间盘 - 椎体单元进行了有意控制的脱水处理，以增强白色不透明纤维束与半透明基质之间的视觉对比度。研究了纤维环结构在圆周和径向位置上的变化。对以下主要结构特征进行了量化：1）除过渡区外，纤维环由15至25个不同的层组成，具体层数取决于圆周位置、脊柱节段和标本年龄；2）在任何20度的圆周扇形区域内，近一半的层终止或起始，从而导致局部层状不规则；3）在这些不规则处有两种可识别的层中断机制；4）各层的厚度在圆周和径向上均有变化，且随年龄显著增加；5）椎间盘总高度上的纤维束数量从20至62不等，平均束间距为0.22毫米。
