Koyalta Donato, Jenabian Mohammad-Ali, Nadjiouroum Ngamasra, Djouater Barou, Djemadji-Oudjeil Noël, Ndjoyi-Mbiguino Angélique, Bélec Laurent
Ministère de la Santé Publique, N'Djamena BP 407, Chad.
BMC Res Notes. 2013 Apr 30;6:169. doi: 10.1186/1756-0500-6-169.
Validation of new affordable CD4 T cell measurement technologies is crucial specifically in resource-poor countries for antiretroviral treatment eligibility and immunologic CD4 monitoring of HIV-infected patients.
The absolute and percentage CD4 T cell counts of 258 HIV-1-infected blood samples (182 adults and 76 children), living in N'Djamena, Chad, were performed by single-platform, volumetric, CD45-assisted pan-leucogating Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) comparing to the FACSCalibur flow cytometer as a reference method.
Absolute and percentage CD4 T cell counts obtained by Auto40 and FACSCalibur of 258 HIV-1-infected blood samples were highly correlated (r = 0.99 and r = 0.96, respectively). The mean absolute bias and percent bias between Apogee Auto40 and FACSCalibur absolute CD4 T cell counts, were -9.4 cells/μl with limits of agreement from -15 to 93 cells/μl, and +2.0% with limits of agreement from -0.9 to 4.9%, respectively. The mean of absolute bias and percent bias between Apogee Auto40 and FACSCalibur of CD4 percentage results were +0.4% (95% CI: -0.02 - 0.86) with limits of agreement from -2.4 to 0.3%, and +3.0% with limits of agreement from -6.6 to 0.6%, respectively. The Auto40 counting allowed to identify the majority of adults with CD4 T cells below 200 cells/μl (sensitivity: 89%; specificity: 99%) or below 350 cells/μl (sensitivity: 94%; specificity:98%); and of children below 750 cells/μl (sensitivity: 99%; specificity: 96%) or below 25% CD4+ (sensitivity: 94%; specificity: 98%).
The Auto40 analyzer is an alternative flow cytometer for CD4 T lymphocyte enumeration to be used in routine for immunological monitoring according to the current WHO recommendations in HIV-infected adults as well as children living in resource-constrained settings like Chad.
新型经济适用的CD4 T细胞检测技术的验证至关重要，特别是在资源匮乏国家，对于确定抗逆转录病毒治疗资格以及对HIV感染患者进行免疫CD4监测而言。
使用单平台、体积法、CD45辅助全白细胞设门的Auto40流式细胞仪（英国赫默尔亨普斯特德的Apogee Flow Systems Ltd公司）对居住在乍得恩贾梅纳的258份HIV-1感染血样（182名成人和76名儿童）进行CD4 T细胞绝对计数和百分比计数，并与FACSCalibur流式细胞仪作为参考方法进行比较。
通过Auto40和FACSCalibur对258份HIV-1感染血样获得的CD4 T细胞绝对计数和百分比计数高度相关（分别为r = 0.99和r = 0.96）。Apogee Auto40与FACSCalibur的CD4 T细胞绝对计数之间的平均绝对偏差和百分比偏差分别为-9.4个细胞/μl，一致性界限为-15至93个细胞/μl，以及+2.0%，一致性界限为-0.9至4.9%。Apogee Auto40与FACSCalibur的CD4百分比结果之间的平均绝对偏差和百分比偏差分别为+0.4%（95%CI：-0.02至0.86），一致性界限为-2.4至0.3%，以及+3.0%，一致性界限为-6.6至0.6%。Auto40计数能够识别出大多数CD4 T细胞低于200个细胞/μl（敏感性：89%；特异性：99%）或低于350个细胞/μl（敏感性：94%；特异性：98%）的成人；以及CD4 T细胞低于750个细胞/μl（敏感性：99%；特异性：96%）或CD4+低于25%（敏感性：94%；特异性：98%）的儿童。
根据世界卫生组织目前针对HIV感染成人及生活在乍得等资源有限环境中的儿童的建议，Auto40分析仪是一种可用于常规免疫监测的CD4 T淋巴细胞计数的替代流式细胞仪。
