Mbopi-Kéou François-Xavier, Mion Stefano, Sagnia Bertrand, Bélec Laurent
Laboratoire National de Santé Hygiène Mobile, Ministère de Santé Publique, Yaoundé, Cameroon, and Faculté de Médecine et des Sciences Biomédicales, Université de Yaoundé I, Yaoundé, Cameroon.
Clin Vaccine Immunol. 2012 Apr;19(4):609-15. doi: 10.1128/CVI.00023-12. Epub 2012 Feb 15.
The study evaluated the single-platform, volumetric, CD45-assisted PanLeucogating Auto40 flow cytometer (Apogee Flow Systems Ltd., Hemel Hempstead, United Kingdom) for CD4 T cell numeration, compared to the reference FACSCalibur flow cytometer. Results of absolute counts and percentages of CD4 T cells by Auto40 and FACSCalibur of 234 tripotassium EDTA (K3-EDTA)-blood samples from 146 adults and 88 children (aged from 18 months to 5 years), living in Yaoundé, Cameroon, were highly correlated (r(2) = 0.97 and r(2) = 0.98, respectively). The mean absolute bias and relative bias between Apogee Auto40 and FACSCalibur absolute CD4 T cell counts were +9.6 cells/μl, with limits of agreement from -251 to 270 cells/μl, and +4.1%, with limits of agreement from -16.1 to 24.4%, respectively. The mean absolute bias and relative bias between Apogee Auto40 and FACSCalibur CD4 T cell results expressed as percentages were +0.05% CD4 (95% confidence interval [CI], -0.03 to 0.41), with limits of agreement from -6.0 to 5.9% CD4, and +1.0%, with limits of agreement from -32.3 to 34.4%, respectively. The Auto40 counting allowed identification of the majority of adults with CD4 T cell counts below 200 cells/μl (sensitivity, 87%; specificity, 98%) or below 350 cells/μl (sensitivity, 92%; specificity, 98%) and of children with CD4 T cell counts below 750 cells/μl (sensitivity, 82%; specificity, 98%) or below 25% CD4(+) (sensitivity, 96%; specificity, 99%). The Auto40 analyzer is a reliable alternative flow cytometer for CD4 T lymphocyte enumeration to be used in routine immunological monitoring according to the WHO recommendations for HIV-infected adults as well as children living in resource-constrained settings.
该研究评估了单平台、体积法、CD45辅助的PanLeucogating Auto40流式细胞仪（英国赫默尔亨普斯特德的Apogee Flow Systems有限公司）用于CD4 T细胞计数的性能，并与参考FACSCalibur流式细胞仪进行了比较。对来自喀麦隆雅温得的146名成人和88名儿童（年龄从18个月至5岁）的234份乙二胺四乙酸三钾（K3-EDTA）血样，采用Auto40和FACSCalibur检测CD4 T细胞的绝对计数和百分比，结果显示二者高度相关（r(2)分别为0.97和0.98）。Apogee Auto40与FACSCalibur的CD4 T细胞绝对计数之间的平均绝对偏差和相对偏差分别为+9.6个细胞/μl，一致性界限为-251至270个细胞/μl，以及+4.1%，一致性界限为-16.1至24.4%。以百分比表示的Apogee Auto40与FACSCalibur的CD4 T细胞结果之间的平均绝对偏差和相对偏差分别为CD4 +0.05%（95%置信区间[CI]，-0.03至0.41），一致性界限为-6.0至5.9% CD4，以及+1.0%，一致性界限为-32.3至34.4%。Auto40计数能够识别出大多数CD4 T细胞计数低于200个细胞/μl（敏感性87%；特异性98%）或低于350个细胞/μl（敏感性92%；特异性98%）的成人，以及CD4 T细胞计数低于750个细胞/μl（敏感性82%；特异性98%）或CD4(+)低于25%（敏感性96%；特异性99%）的儿童。根据世界卫生组织针对资源有限环境中感染HIV的成人及儿童的建议，Auto40分析仪是用于CD4 T淋巴细胞计数的可靠替代流式细胞仪，可用于常规免疫监测。
