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[一种基于槲皮素氧化反应的简单灵敏的超氧化物歧化酶活性测定方法]

[A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation].

作者信息

Kostiuk V A, Potapovich A I, Kovaleva Zh V

出版信息

Vopr Med Khim. 1990 Mar-Apr;36(2):88-91.

PMID:2363268
Abstract

An indirect method for determination of superoxide dismutase (SOD) activity, based on inhibition of quercetin autooxidation, was developed. The optimal conditions of reaction ensuring high sensitivity of measurement (I50 = 1.5 ng/ml) were selected. NaN3, catalase and physiological concentrations of ascorbic acid did not interfere with the SOD estimation in blood and tissue homogenates.

摘要

基于抑制槲皮素自氧化反应，开发了一种间接测定超氧化物歧化酶（SOD）活性的方法。选择了确保测量具有高灵敏度（I50 = 1.5 ng/ml）的最佳反应条件。NaN3、过氧化氢酶和生理浓度的抗坏血酸不干扰血液和组织匀浆中SOD的测定。

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