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Superoxide--driven oxidation of quercetin and a simple sensitive assay for determination of superoxide dismutase.

作者信息

Kostyuk V A, Potapovich A I

机构信息

Department of Biology, V. I. Lenin Byelorussion State University, Minsk, BSSR, USSR.

出版信息

Biochem Int. 1989 Nov;19(5):1117-24.

PMID:2561443
Abstract

Oxidation of quercetin at pH 10 was shown to be a free radical chain reaction involving superoxide and hence inhibitable by superoxide dismutase (SOD) (EC 1.15.1.1). The degree of inhibition of quercetin oxidation was a function of SOD concentration, and fifty percent inhibition was produced by approximately 1.5 ng/ml of pure enzyme. This reaction proved to be a very useful tool for a rapid and highly sensitive measurement of SOD in crude tissue extracts and other biological samples.

摘要

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