Ko Chang-Yong, Jung Young Jin, Seo Dong Hyeon, Kim Han Sung
Research Team, Korea Orthopedics & Rehabilitation Engineering Center, Incheon, Republic of Korea.
Proc Inst Mech Eng H. 2013 Jul;227(7):815-20. doi: 10.1177/0954411913485053. Epub 2013 Apr 18.
This study aimed to determine whether there is bilateral asymmetry between the left and right tibiae in the microarchitectural characteristics of the trabecular bone and estimate a moderate sample size for detections of such bilateral differences. The left and right tibiae of 20 C57BL/6 mice (12 weeks old) were scanned by micro-computed tomography, and the structural parameters of the trabecular bone were measured. There were significant differences in the structural parameters between the left and right tibiae (p<0.05); specifically, the microarchitecture was greater in quantity and quality in the left tibia than in the right tibia. Moreover, the percent of bilateral differences between the left and right tibiae ranged from 2.26% to 22.52%. For most of the structural parameters except for trabecular bone thickness (Tb.Th) and trabecular bone separation (Tb.Sp), the 20 mice involved in this study were enough to detect differences. These results show that the directional left-right asymmetry in the microarchitecture of the trabecular bone may exist despite the samples being from an inbred strain. Furthermore, we estimated a sample size for detections of such differences between the left and right tibiae.
本研究旨在确定小梁骨微结构特征在左右胫骨之间是否存在双侧不对称，并估计检测此类双侧差异所需的适度样本量。对20只12周龄C57BL/6小鼠的左右胫骨进行了显微计算机断层扫描，并测量了小梁骨的结构参数。左右胫骨的结构参数存在显著差异（p<0.05）；具体而言，左胫骨小梁骨的数量和质量均优于右胫骨。此外，左右胫骨之间的双侧差异百分比在2.26%至22.52%之间。对于除小梁骨厚度（Tb.Th）和小梁骨间距（Tb.Sp）之外的大多数结构参数，本研究中涉及的20只小鼠足以检测到差异。这些结果表明，尽管样本来自近交系，但小梁骨微结构中可能存在左右方向的不对称。此外，我们估计了检测左右胫骨之间此类差异所需的样本量。
