Iscan M, Reuhl K, Weiss B, Maines M D
Department of Biophysics and Environmental Health Sciences Center, University of Rochester, NY 14642.
Biochem Biophys Res Commun. 1990 Jun 29;169(3):858-63. doi: 10.1016/0006-291x(90)91972-u.
In brain of female monkey (M. fascicularis) the content of cytochrome P-450 and benzo(a)pyrene hydroxylase activity in the mitochondrial fraction exceeded that of the microsomes by more than 4-fold. The mitochondrial drug metabolism activity exhibited substrate specificity and, unlike the microsomes, did not catalyze 7-ethoxyresorufin O-deethylase reaction. Moreover, the rate of benzo(a)pyrene hydroxylase activity by both the mitochondrial and the microsomal fractions displayed regional variation with the olfactory bulb displaying the highest hydroxylase activity. In contrast, the microsomal 7-ethoxyresorufin O-deethylase activity was uniformally distributed in all brain regions.
在雌性食蟹猴的大脑中，线粒体部分的细胞色素P-450含量和苯并(a)芘羟化酶活性比微粒体部分高出4倍多。线粒体药物代谢活性表现出底物特异性，与微粒体不同，它不催化7-乙氧基异吩恶唑酮O-脱乙基酶反应。此外，线粒体和微粒体部分的苯并(a)芘羟化酶活性速率均呈现区域差异，嗅球的羟化酶活性最高。相比之下，微粒体7-乙氧基异吩恶唑酮O-脱乙基酶活性在所有脑区均一分布。
