MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom.
PLoS One. 2013 Apr 29;8(4):e62054. doi: 10.1371/journal.pone.0062054. Print 2013.
There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models.
目前对更高效的转基因构建体的需求越来越大。在此，我们介绍了一种通用的多克隆位点 Gateway 载体，可用于重组酶反应。通过转基因小鼠模型，我们展示了它在 BAC 转基因和靶向载体生成中的应用。该载体的模块化特性允许快速修改构建体，以生成同一构建体的不同版本。因此，它将有助于简化一系列相关转基因模型的生成。
