The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
Vet Comp Oncol. 2013 Jun;11(2):113-23. doi: 10.1002/vco.306.
Malignant melanoma (MM) is one of the most aggressive cancers in dogs and in humans. However, the molecular mechanisms of its development and progression remain unclear. Presently, we examined the expression profile of microRNAs (miRs) in canine oral MM tissues and paired normal oral mucosa tissues by using the microRNA-microarray assay and quantitative RT-PCR. Importantly, a decreased expression of miR-203 was significantly associated with a shorter survival time. Also, miR-203 and -205 were markedly down-regulated in canine and human MM cell lines tested. Furthermore, the ectopic expression of miR-205 had a significant inhibitory effect on the cell growth of canine and human melanoma cells tested by targeting erbb3. Our data suggest that miR-203 is a new prognostic factor in canine oral MMs and that miR-205 functions as a tumour suppressor by targeting erbb3 in both canine and human MM cells.
恶性黑色素瘤（MM）是犬和人类中最具侵袭性的癌症之一。然而，其发展和进展的分子机制尚不清楚。目前，我们通过 microRNA-microarray 检测和定量 RT-PCR 分析，检测了犬口腔 MM 组织和配对的正常口腔黏膜组织中 microRNAs (miRs) 的表达谱。重要的是，miR-203 的表达降低与生存时间较短显著相关。此外，在测试的犬和人 MM 细胞系中，miR-203 和 miR-205 明显下调。此外，miR-205 的异位表达通过靶向 erbb3 对测试的犬和人黑素瘤细胞的细胞生长具有显著的抑制作用。我们的数据表明，miR-203 是犬口腔 MM 的一个新的预后因素，miR-205 通过靶向 erbb3 在犬和人 MM 细胞中发挥肿瘤抑制作用。
