Department of Physics.
Int J Radiat Biol. 2013 Oct;89(10):765-9. doi: 10.3109/09553002.2013.800245. Epub 2013 May 22.
In a previous paper, we showed that chemiluminescence from radical recombination (initiated by lipid peroxidation and propagated by polyunsaturated fatty acids [PUFA]) has a bleaching effect comparable to that caused by light on the rhodopsin of retinal rod outer segment (RdOS) prepared from bovine eyes. Photons generated by radical recombination were suggested to be the origin of phosphenes perceived as light flashes by the human eye. Irradiation with (12)C carbon ions was used in this study to stimulate radical production, propagation and recombination leading to photoluminescence.
(12)C radiation bleached RdOS rhodopsin, but structural damage increasing with the radiation dose was also observed. For this reason, only the effects on rhodopsin at doses producing next to negligible biodamage and permitting regeneration have been considered as bleaching effects.
(12)C irradiation bleached RdOS rhodopsin, but increasing structural damage with radiation dose was also observed. For the measure of bleaching and to reveal dose response effects on rhodopsin that were able to be regenerated only results from doses producing nearly negligible biodamage have been considered.
Recombination of radicals appears responsible for the release of photons with subsequent bleaching of rhodopsin. This effect could have an important role in the generation of the anomalous visual effects (phosphenes) experienced by patients during hadrotherapy or by astronauts in space.
在之前的一篇论文中，我们表明自由基复合（由脂质过氧化引发并由多不饱和脂肪酸[PUFA]传播）产生的化学发光具有与光对从牛眼制备的视网膜外节（RdOS）视紫红质造成的漂白效果相当。自由基复合产生的光子被认为是人类眼睛感知到的闪光幻视的来源。本研究中使用（12）C 碳离子辐照来刺激自由基的产生、传播和复合，从而产生光致发光。
（12）C 辐射使 RdOS 视紫红质漂白，但也观察到结构损伤随辐射剂量的增加而增加。因此，仅考虑了在产生几乎可以忽略不计的生物损伤且允许再生的剂量下对视紫红质的影响作为漂白效应。
（12）C 辐照使 RdOS 视紫红质漂白，但也观察到结构损伤随辐射剂量的增加而增加。为了测量漂白并揭示仅能再生的视紫红质对剂量的响应效应，仅考虑了在产生几乎可以忽略不计的生物损伤的剂量下的结果。
自由基的复合似乎负责释放随后漂白视紫红质的光子。这种效应在放射性治疗期间患者或宇航员在太空中经历的异常视觉效应（闪光幻视）的产生中可能具有重要作用。
