Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, Daejeon 305-701, Korea.
Nano Lett. 2013 Jun 12;13(6):2431-5. doi: 10.1021/nl4003393.
Delivery of bioactive materials into a cell is highly important in the study of cell biology and medical treatments. Ideal nanoinjectors should be able to deliver biomaterials with high spatial resolution while causing minimum cell damage. We developed a Au nanowire (NW) nanoinjector that has the thinnest diameter (100–150 nm) among the DNA delivering devices as well as optimum mechanical properties, minimizing cell damage. Well-defined (111) single-crystalline Au surface and high electric conductivity of a Au NW nanoinjector allow precisely timed and efficient electrochemical release of DNA molecules attached on a Au NW surface. Both linear DNA and plasmid DNA were delivered separately and showed successful expression. The Au NW nanoinjector would find important biomedical applications in the fields such as gene therapy, DNA vaccination, targeted drug delivery, and probe/control of cell signaling events.
将生物活性物质递送到细胞内对于细胞生物学和医学治疗的研究非常重要。理想的纳米注射器应该能够以高空间分辨率递送生物材料，同时将细胞损伤降到最低。我们开发了一种金纳米线（NW）纳米注射器，它是具有最细直径（100-150nm）的 DNA 输送装置之一，同时具有最佳的机械性能，可将细胞损伤降到最低。金纳米线注射器具有明确的（111）单晶金表面和高导电性，可精确控制时间并高效电化学释放附着在金纳米线表面的 DNA 分子。线性 DNA 和质粒 DNA 都被分别递送到细胞内，并成功表达。金纳米线纳米注射器将在基因治疗、DNA 疫苗接种、靶向药物输送以及探针/细胞信号事件控制等领域找到重要的生物医学应用。
