Pomponi Shirley A, Jevitt Allison, Patel Jignasa, Diaz M Cristina
*Harbor Branch Oceanographic Institute, Florida Atlantic University, Fort Pierce, FL 34946, USA; Florida State University, Tallahassee, FL 32306, USA; Oceanographic Center, Nova Southeastern University, Dania Beach, FL 33004, USA.
Integr Comp Biol. 2013 Sep;53(3):524-30. doi: 10.1093/icb/ict032. Epub 2013 May 2.
Many sponge-derived natural products with applications to human health have been discovered over the past three decades. In vitro production has been proposed as one biological alternative to ensure adequate supply of marine natural products for preclinical and clinical development of drugs. Although primary cell cultures have been established for many marine phyla, no cell lines with an extended life span have been established for marine invertebrates. Hybridoma technology has been used for production of monoclonal antibodies for application to human health. We hypothesized that a sponge cell line could be formed by fusing sponge cells of one species with those of another, or by fusing sponge cells with rapidly dividing, marine-derived, non-sponge cells. Using standard methods for formation of hybridomas, with appropriate modifications for temperature and salinity, cells from individuals of the same sponge species, as well as cells from individuals of two different sponge species were successfully fused. Research in progress is focused on optimizing fusion to produce a cell line and to stimulate expression of natural products with therapeutic relevance. Experimental hybridomas may also be used as models to test hypotheses related to naturally occurring sponge chimeras and hybridomas.
在过去三十年中，已发现许多源自海绵的天然产物可应用于人类健康领域。体外生产已被提议作为一种生物学替代方法，以确保为药物的临床前和临床开发提供充足的海洋天然产物供应。尽管已为许多海洋门类建立了原代细胞培养物，但尚未为海洋无脊椎动物建立具有延长寿命的细胞系。杂交瘤技术已用于生产应用于人类健康的单克隆抗体。我们假设，可以通过将一种海绵的细胞与另一种海绵的细胞融合，或者通过将海绵细胞与快速分裂的、源自海洋的非海绵细胞融合来形成海绵细胞系。使用形成杂交瘤的标准方法，并对温度和盐度进行适当修改，成功融合了来自同一海绵物种个体的细胞以及来自两种不同海绵物种个体的细胞。正在进行的研究重点是优化融合以产生细胞系，并刺激具有治疗相关性的天然产物的表达。实验性杂交瘤也可用作模型，以检验与天然存在的海绵嵌合体和杂交瘤相关的假设。
