Department of Urology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
J Transl Med. 2013 May 4;11:111. doi: 10.1186/1479-5876-11-111.
Special AT-rich sequence binding protein 1 (SATB1) is a nuclear factor that functions as the global chromatin organizer to regulate chromatin structure and gene expression gene expression. SATB1 has been shown to be abnormally expressed in various types of cancer. However, the expression and role of SATB1 in prostate cancer remain unclear.
120 cases of prostatic carcinoma and 60 cases of benign prostate hyperplasia were analyzed for SATB1 expression by immunohistochemistry. LNCaP, DU-145, and PC3 prostate cancer cells were examined for SATB1 expression by Western blot analysis. Cell proliferation and invasion was evaluated by CCK8 and transwell invasion assay, respectively.
SATB1 staining was stronger in prostatic carcinomas with metastasis than in those without metastasis, but was absent in benign prostate hyperplasia. Furthermore, SATB1 expression was positively correlated with bone metastasis and the Gleason score. SATB1 overexpression promoted the proliferation and invasion of LNCaP cells while SATB1 knockdown inhibited the proliferation and invasion of DU-145 cells.
These findings provide novel insight into oncogenic role of SATB1 in prostate cancer, suggesting that SATB1 is a promising biomarker and therapeutic target for prostate cancer.
特殊 AT 富含序列结合蛋白 1（SATB1）是一种核因子，作为全局染色质组织者发挥作用，调节染色质结构和基因表达。SATB1 在各种类型的癌症中表达异常。然而，SATB1 在前列腺癌中的表达和作用仍不清楚。
通过免疫组织化学分析了 120 例前列腺癌和 60 例良性前列腺增生患者的 SATB1 表达。通过 Western blot 分析检测 LNCaP、DU-145 和 PC3 前列腺癌细胞中的 SATB1 表达。通过 CCK8 和 Transwell 侵袭实验分别评估细胞增殖和侵袭。
有转移的前列腺癌中 SATB1 染色比无转移的更强，但良性前列腺增生中没有。此外，SATB1 表达与骨转移和 Gleason 评分呈正相关。SATB1 过表达促进了 LNCaP 细胞的增殖和侵袭，而 SATB1 敲低抑制了 DU-145 细胞的增殖和侵袭。
这些发现为 SATB1 在前列腺癌中的致癌作用提供了新的见解，表明 SATB1 是前列腺癌有前途的生物标志物和治疗靶点。
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