The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
Ecotoxicol Environ Saf. 2013 Jul;93:22-30. doi: 10.1016/j.ecoenv.2013.03.036. Epub 2013 May 2.
This study is aimed at evaluating the effects of endosulfan in clams (Ruditapes philippinarum). For this purpose, a study was performed on clams exposed to 0.005, 0.05 and 0.5μg/L endosulfan for 15 days. S ubsequently, the level of ethoxyresorufin-O-deethylase (EROD) activity, glutathione-S-transferase (GST) activity, glutathione (GSH) content, superoxide dismutase (SOD) activity, lipid peroxidation (LPO) and DNA strand break was determined in gills and digestive glands. Among the parameters, endosulfan caused significant changes in induction of EROD activity and oxidative stress in clams R. philippinarum. The exposure to endosulfan increased the concentration of EROD, GST, GSH, MDA and decreased the concentration of SOD. Moreover, according to the correlation analysis results, the EROD activity and GSH content in digestive gland as well as GST activity, LPO and DNA damage in both tissues had excellent correlation with endosulfan concentration. These results provided information on potential biomarkers that could be effectively applied to the biomonitoring of aquatic ecosystem in areas susceptible to persistent organochlorine compounds contamination, and also information on toxic effects.
本研究旨在评估硫丹对蛤（菲律宾蛤仔）的影响。为此，对暴露于 0.005、0.05 和 0.5μg/L 硫丹中的蛤进行了为期 15 天的研究。随后，测定了鳃和消化腺中乙氧基试卤灵-O-去乙基酶（EROD）活性、谷胱甘肽-S-转移酶（GST）活性、谷胱甘肽（GSH）含量、超氧化物歧化酶（SOD）活性、脂质过氧化（LPO）和 DNA 链断裂。在这些参数中，硫丹导致菲律宾蛤仔 R. philippinarum 的 EROD 活性和氧化应激诱导发生显著变化。暴露于硫丹增加了 EROD、GST、GSH、MDA 的浓度，降低了 SOD 的浓度。此外，根据相关分析结果，消化腺中的 EROD 活性和 GSH 含量以及两种组织中的 GST 活性、LPO 和 DNA 损伤与硫丹浓度具有极好的相关性。这些结果为在易受持久性有机氯化合物污染的地区的水生生态系统生物监测中有效应用潜在生物标志物提供了信息，同时也提供了有关毒性影响的信息。
