Department of Chemistry, National Sun Yat - Sen University, Kaohsiung 80424, 70, Lien-Hai Road, Taiwan.
Biomaterials. 2013 Jul;34(23):5833-42. doi: 10.1016/j.biomaterials.2013.03.077. Epub 2013 Apr 30.
This study demonstrates the effective synthesis of five different sized/shaped Pt NPs, within a narrow size regime of 1-21 nm using a modified methodology and the toxicity/biocompatibility of Pt NPs on Neuro 2A cancer cells was investigated elaborately by using light microscopic observations, tryphan blue exclusion assay, MTT assay and ICP-MS. The Pt NPs-C with sizes 5-6 nm showed superior non-cytotoxic property compared to the other four Pt NPs. These non-cytotoxic Pt NPs were employed for successful photothermal treatment of Neuro 2A cell lines using near-IR 1064 nm of laser irradiation. The Pt NPs-C could generate a 9 °C increase in temperature leading to effective photothermal killing of cancer cells. The MALDI-MS was used to prove the possibility of apoptosis related triggering of cell death in the presence of the Pt NPs. The results confirm that the current approach is an effective platform for in vivo treatment of neuro cancer cells.
本研究使用改良的方法成功合成了五种不同尺寸/形状的 Pt NPs，尺寸范围在 1-21nm 之间。通过使用光学显微镜观察、台盼蓝排斥试验、MTT 试验和 ICP-MS，详细研究了 Pt NPs 对 Neuro 2A 癌细胞的毒性/生物相容性。尺寸为 5-6nm 的 Pt NPs-C 表现出优于其他四种 Pt NPs 的优异非细胞毒性特性。这些非细胞毒性 Pt NPs 成功地用于使用近红外 1064nm 激光照射对 Neuro 2A 细胞系进行光热治疗。Pt NPs-C 能够产生 9°C 的温升，从而有效杀死癌细胞。MALDI-MS 被用来证明在 Pt NPs 的存在下细胞死亡与细胞凋亡相关的触发的可能性。结果证实，目前的方法是治疗神经癌细胞的有效体内治疗平台。
